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* '''Description:''' fructose-specific [[PTS|phosphotransferase system]], EIID component of the [[PTS]]<br/><br/>
{{#bank: LevG}}


{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''levG''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || fructose-specific [[PTS|phosphotransferase system]]<br/>EIID component
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27040 levG]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=LevG LevG]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=levG levG]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 9.935 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 825 bp, 275 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacC]]'', ''[[levF]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27040 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27040 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27040 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:levG_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levG_2760233_2761060_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:levG_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27040]]
|-
|}
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= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[utilization of specific carbon sources]]}}
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}},
{{SubtiWiki regulon|[[LevR regulon]]}},
{{SubtiWiki regulon|[[SigL regulon]]}}
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU27040
===Phenotypes of a mutant ===
=== Database entries ===
* '''DBTBS entry:''' no entry
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10319]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:'''
* '''Protein family:''' [[PTS]] permease, mannose permease (Man) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''[[SubtInteract|Interactions]]:''' [[LevF]]-[[LevG]]
* '''[[Localization]]:'''
=== Database entries ===
* '''Structure:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/P26382 P26382]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27040]
* '''E.C. number:'''
=== Additional information===
=Expression and regulation=
* '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levG_2760233_2761060_-1 levG] {{PubMed|22383849}}
* '''Sigma factor:''' [[SigL]] {{PubMed|1924373}}
* '''Regulation:'''
** subject to carbon catabolite repression ([[CcpA]], [[LevR]]) {{PubMed|7592486}}
** induced in the presence of fructose ([[LevR]]) {{PubMed|1900939}}
* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression {{PubMed|7592486}}
** [[LevR]]: transcription activation {{PubMed|1900939}}
* '''Additional information:'''
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
<pubmed>10627040 2117666, 1924373 7592486 1900939</pubmed>
[[Category:Protein-coding genes]]

Latest revision as of 14:20, 2 April 2014