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SacC: Difference between revisions

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* '''Description:''' levanase <br/><br/>
{{#bank: SacC}}


{| align="right" border="1" cellpadding="2"
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|style="background:#ABCDEF;" align="center"| '''Product''' || levanase
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|style="background:#ABCDEF;" align="center"|'''Function''' || degradation of levan to fructose
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27030 sacC]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sacC sacC]'''
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 75 kDa, 6.789 
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2031 bp, 677 aa
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yraA]]'', ''[[levG]]''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27030 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27030 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27030 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:sacC_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacC_2758043_2760076_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sacC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27030]]
|-
|}
__TOC__
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= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}}
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}},
{{SubtiWiki regulon|[[LevR regulon]]}},
{{SubtiWiki regulon|[[SigL regulon]]}}
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU27030
===Phenotypes of a mutant ===
=== Database entries ===
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/levDEFG-sacC.html]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10320]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:''' Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to Swiss-Prot)
* '''Protein family:''' glycosyl hydrolase 32 family (according to Swiss-Prot)
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''[[SubtInteract|Interactions]]:'''
* '''[[Localization]]:'''
=== Database entries ===
* '''Structure:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/P05656 P05656]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27030]
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.80 3.2.1.80]
=== Additional information===
=Expression and regulation=
* '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacC_2758043_2760076_-1 sacC] {{PubMed|22383849}}
* '''[[Sigma factor]]:''' [[SigL]] {{PubMed|1924373}}
* '''Regulation:'''
** subject to carbon catabolite repression ([[CcpA]], [[LevR]]) {{PubMed|7592486}}
** induced in the presence of fructose ([[LevR]]) {{PubMed|1900939}}
* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression {{PubMed|7592486}}
** [[LevR]]: transcription activation {{PubMed|1900939}}
* '''Additional information:'''
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
==Reviews==
<pubmed>20735481 </pubmed>
==Original publications==
Additional publications: {{PubMed|23213210}}
<pubmed>3112519 2117666, 19635162 1900939 1924373 7592486 </pubmed>
[[Category:Protein-coding genes]]

Latest revision as of 14:20, 2 April 2014