BkdR

• Description: transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon
  
  

Gene name	bkdR
Synonyms	yqiR
Essential	no
Product	transcriptional activator (for SigL-dependent promoter))
Function	regulation of branched-chain amino acid utilization, required for survival at low temperatures)
Gene expression levels in SubtiExpress: bkdR required for survival at low temperatures)
Interactions involving this protein in SubtInteract: BkdR
Metabolic function and regulation of this protein in SubtiPathways: bkdR
MW, pI	77 kDa, 6.237
Gene length, protein length	2076 bp, 692 aa
Immediate neighbours	ptb, yqzF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

CodY regulon

The BkdR regulon:

• ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

The gene

Basic information

• Locus tag: BSU24100

Phenotypes of a mutant

The mutant is cold-sensitive PubMed

Database entries

• BsubCyc: BSU24100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: BkdR-SigL (activation)

• Localization:

Database entries

• BsubCyc: BSU24100

• Structure:

• UniProt: P54529

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bkdR PubMed

• Expression browser: bkdR PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A913 ( bkdR::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>16585774,10094682, 23123912,12823818 , </pubmed>