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| * '''Description:''' 5-amino-6-(5-phosphoribosylamino)uracil reductase <br/><br/>
| | {{#bank: RibD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ribD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ribG ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || 5-amino-6-(5-phosphoribosylamino)uracil reductase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || riboflavin biosynthesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23280 ribD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ribD ribD]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 6.079
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1083 bp, 361 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ribE]]'', ''[[ypuD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23280 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23280 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23280 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ribD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ribD_2430258_2431343_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ribD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23280]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis of cofactors]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[FMN-box]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU23280
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ypuE-ribDEAHT.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10518]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H<sub>2</sub>O = 5-amino-6-(5-phosphoribosylamino)uracil + NH<sub>3</sub> (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EX8 3EX8] {{PubMed|18986985}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P17618 P17618]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23280]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.5.4.26 3.5.4.26], [http://www.expasy.org/enzyme/1.1.1.193 1.1.1.193]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ribD]]-[[ribE]]-[[ribA]]-[[ribH]]-[[ribT]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8159171 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ribD_2430258_2431343_-1 ribD] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8159171 PubMed]
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| * '''Regulation:''' expressed in the absence of FMN ([[FMN-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15808508 PubMed]
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| * '''Regulatory mechanism:''' [[FMN-box]]: [[riboswitch]], mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination [http://www.ncbi.nlm.nih.gov/sites/entrez/15808508 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|23385458}}
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| <pubmed>12456892,15808508, 18986985, 8159171, 7934830 23270261 </pubmed>
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| [[Category:Protein-coding genes]]
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