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| * '''Description:''' DNA polymerase III (alpha subunit), part of the [[replisome]] <br/><br/>
| | {{#bank: PolC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''polC''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''mutI, dnaF, dnaP ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || DNA polymerase III (alpha subunit)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16580 polC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PolC PolC]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 162 kDa, 5.135
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 4311 bp, 1437 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[proS]]'', ''[[ylxS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16580 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16580 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16580 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:polC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=polC_1727133_1731446_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:polC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16580]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA replication]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16580
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10263]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
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| ** required for bacteriophage SPP1 replication {{PubMed|23268446}}
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| * '''Protein family:''' PolC subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}}
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| ** [[DnaE]]-[[PolC]] {{PubMed|23563155}}
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| * '''[[Localization]]:'''
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| ** forms foci {{PubMed|21958350}}
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| ** cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| ** [http://www.pdb.org/pdb/explore/explore.do?structureId=3f2b 3F2B] (from ''Geobacillus kaustophilus'', 71% identity) {{PubMed|19106298}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P13267 P13267]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16580]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.7 2.7.7.7]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=polC_1727133_1731446_1 polC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 19106294</pubmed>
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| == Original publications ==
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| <pubmed>15186423, 20122408,11721055 23563155 23268446,21740522,21958350 24062730 24106089 19106298</pubmed>
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| [[Category:Protein-coding genes]]
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