|
|
| Line 1: |
Line 1: |
| * '''Description:''' spore coat protein (insoluble fraction), necessary for the assembly of the spore crust, the outermost layer of the spore coat<br/><br/>
| | {{#bank: CotX}} |
| {| align="right" border="1" cellpadding="2" | |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''cotX''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (insoluble fraction)
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || spore coat assembly
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11760 cotX]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 4.228
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 516 bp, 172 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cotY]]'', ''[[cotW]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11760 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:cotX_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotX_1250656_1251174_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cotX_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11760]]
| |
| |-
| |
| |}
| |
|
| |
|
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[sporulation proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[GerE regulon]]}},
| |
| {{SubtiWiki regulon|[[SigK regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU11760
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cotVWX.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10500]
| |
|
| |
| === Additional information===
| |
| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
|
| |
| * '''Protein family:'''
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** spore crust {{PubMed|23202530}}
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q08313 Q08313]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11760]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[cotV]]-[[cotW]]-[[cotX]]'' [http://www.ncbi.nlm.nih.gov/pubmed/7519271 PubMed]
| |
|
| |
| ''cotX'' [http://www.ncbi.nlm.nih.gov/pubmed/7519271 PubMed]
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotX_1250656_1251174_-1 cotX] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' before ''[[cotV]]'': [[SigK]] {{PubMed|15699190,7519271}}
| |
|
| |
| before ''[[cotX]]'': [[SigK]] [http://www.ncbi.nlm.nih.gov/pubmed/7519271 PubMed]
| |
|
| |
| * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
| |
|
| |
| * '''Regulatory mechanism:''' upstream of ''[[cotX]]'': [[SpoIIID]]: repression [http://www.ncbi.nlm.nih.gov/pubmed/10788508 PubMed], upstream of both promoters: [[GerE]]: transcription activation [http://www.ncbi.nlm.nih.gov/pubmed/7519271 PubMed]
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| == Reviews ==
| |
| <pubmed>23202530</pubmed>
| |
| == Original publications ==
| |
| <pubmed>7519271,10788508, 20451384,12107147, 21665972 21821766</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
