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| * '''Description:''' [[ABC transporter]] (permease), export of the spore killing factor <br/><br/>
| | {{#bank: SkfF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''skfF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybdB''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[ABC transporter]] (permease)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || export of the spore killing factor [[SkfA]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01960 skfF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SkfF SkfF]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 51 kDa, 9.222
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1344 bp, 448 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[skfE]]'', ''[[skfG]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01960 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ybdB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=skfF_217697_219040_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:skfF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01960]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ABC transporters]]}},
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| {{SubtiWiki category|[[toxins, antitoxins and immunity against toxins]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[prophage 1]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[PhoP regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU01960
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12719]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SkfE]]-[[SkfF]] {{PubMed|11731129}}
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| * '''[[Localization]]:''' membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31428 O31428]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01960]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[skfA]]-[[skfB]]-[[skfC]]-[[skfE]]-[[skfF]]-[[skfG]]-[[skfH]]'' [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=skfF_217697_219040_1 skfF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/16452424 PubMed]
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| * '''Regulation:'''
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]
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| ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]
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| ** induced by phosphate starvation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]
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| ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]
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| ** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]
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| * '''Additional information:''' Northern blotting during during phosphate limitation showed an intense 0.25 kb ''[[skfA]]''-specific transcript, and a weaker 6.5 kb ''[[skfA]]-[[skfB]]-[[skfC]]-[[skfE]]-[[skfF]]-[[skfG]]-[[skfH]]'' transcript. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20955377 </pubmed>
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| ==Original Publications==
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| <pubmed>10092453,12817086,,16816204,14651647,15687200,15687200,17720793, 11731129 ,17449691,18840696 </pubmed>
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| [[Category:Protein-coding genes]]
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