GamA

• Description: glucosamine-6-phosphate deaminase
  
  

Gene name	gamA
Synonyms	ybfT
Essential	no
Product	glucosamine-6-phosphate deaminase
Function	glucosamine utilization
Gene expression levels in SubtiExpress: gamA
Metabolic function and regulation of this protein in SubtiPathways: gamA
MW, pI	27 kDa, 5.793
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	gamP, gamR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

GamR regulon

The gene

Basic information

• Locus tag: BSU02360

Phenotypes of a mutant

• no growth on glucosamine PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: d-glucosamine 6-phosphate + H₂O = D-fructose 6-phosphate + NH₃ (according to Swiss-Prot)

• Protein family: NagB subfamily (according to Swiss-Prot)

• Paralogous protein(s): NagB

Extended information on the protein

• Kinetic information: K(M): 3.0 mM PubMed

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BKX (the structure of NagB, 50% identity) PubMed

• UniProt: O31458

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: gamA-gamP PubMed

• Expression browser: gamA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by N-acetylglucosamine PubMed
  • induced by glucosamine (GamR) PubMed

• Regulatory mechanism:
  • GamR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>10627040, 14343123 23667565 23876412 24673833,15755726 </pubmed>