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= [[Categories]] containing this gene/protein =
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[sulfur metabolism]]}}
{{SubtiWiki category|[[sulfur metabolism]]}},
[[most abundant proteins]]


= This gene is a member of the following [[regulons]] =
= This gene is a member of the following [[regulons]] =
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=== Additional information===
=== Additional information===


=The protein=
=The protein=
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* '''Kinetic information:'''
* '''Kinetic information:'''


* '''Domains:'''  
* '''[[Domains]]:'''  


* '''Modification:'''
* '''Modification:'''


* '''Cofactor(s):'''
* '''[[Cofactors]]:'''


* '''Effectors of protein activity:'''
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sat_1632208_1633356_1 sat] {{PubMed|22383849}}
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sat_1632208_1633356_1 sat] {{PubMed|22383849}}


* '''Sigma factor:''' [[SigA]] {{PubMed|11004190}}
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11004190}}


* '''Regulation:'''  
* '''Regulation:'''  
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* '''Additional information:'''
* '''Additional information:'''
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}


=Biological materials =
=Biological materials =
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=References=
=References=


<pubmed>16267287,11004190,12107147, 18039762 </pubmed>
<pubmed>16267287,11004190,12107147, 18039762 15378759</pubmed>


[[Category:Protein-coding genes]]
[[Category:Protein-coding genes]]

Revision as of 16:16, 5 March 2014

  • Description: sulfate adenylyltransferase

Gene name sat
Synonyms ylnB
Essential no
Product sulfate adenylyltransferase
Function sulfate activation
Gene expression levels in SubtiExpress: sat
Metabolic function and regulation of this protein in SubtiPathways:
sat
MW, pI 42 kDa, 5.646
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours cysP, cysC
Sequences Protein DNA DNA_with_flanks
Genetic context
This image was kindly provided by SubtiList
Expression at a glance   PubMed















Categories containing this gene/protein

sulfur metabolism, most abundant proteins

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15590

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + sulfate = diphosphate + adenylyl sulfate (according to Swiss-Prot)
  • Protein family: sulfate adenylyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s): YitA

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References

<pubmed>16267287,11004190,12107147, 18039762 15378759</pubmed>