PtkA: Difference between revisions
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=== Additional information=== | === Additional information=== | ||
=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
* '''Domains:''' single BY-kinase domain | * '''[[Domains]]:''' single BY-kinase domain | ||
* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site) {{PubMed|20509597}}, dephosphorylated by [[PtpZ]] {{PubMed|15866923}} | * '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site) {{PubMed|20509597}}, dephosphorylated by [[PtpZ]] {{PubMed|15866923}} | ||
* ''' | * '''[[Cofactors]]:''' ATP | ||
* '''Effectors of protein activity:''' [[TkmA]] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed] | * '''Effectors of protein activity:''' [[TkmA]] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed] | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
** KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]] | ** KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]] | ||
** GP1520 (spc), available in | ** GP1520 (spc), available in [[ Jörg Stülke]]'s lab | ||
** GP1544 (ermC), available in | ** GP1544 (ermC), available in [[ Jörg Stülke]]'s lab | ||
** GP1587 (cat) , available in [[Stülke]] lab | ** GP1587 (cat) , available in [[ Jörg Stülke]]'s lab | ||
** GP1521 epsB (aphA3) ptkA (spc) double mutant available in [[Stülke]] lab | ** GP1521 ''[[epsB]]'' (aphA3) ''[[ptkA]]'' (spc) double mutant available in [[ Jörg Stülke]]'s lab | ||
** GP1529 tkmA-ptkA::spc available in [[Stülke]] lab | ** GP1529 ''[[tkmA]]-[[ptkA]]''::spc available in [[ Jörg Stülke]]'s lab | ||
** GP1610 (ptkA-ptpZ, spc), available in | ** GP1610 (''[[ptkA]]-[[ptpZ]]'', spc), available in [[ Jörg Stülke]]'s lab | ||
* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]] | * '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]] | ||
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* '''GFP fusion:''' CFP-fusion, available from [[Ivan Mijakovic]] | * '''GFP fusion:''' CFP-fusion, available from [[Ivan Mijakovic]] | ||
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Jörg Stülke]]'s lab | ||
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 10:22, 31 January 2014
- Description: protein tyrosine kinase
| Gene name | ptkA |
| Synonyms | ywqD |
| Essential | no |
| Product | protein tyrosine kinase |
| Function | protein phosphorylation |
| Gene expression levels in SubtiExpress: ptkA | |
| Interactions involving this protein in SubtInteract: PtkA | |
| MW, pI | 25 kDa, 9.628 |
| Gene length, protein length | 711 bp, 237 aa |
| Immediate neighbours | ptpZ, tkmA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Categories containing this gene/protein
biofilm formation, protein modification, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36250
Phenotypes of a mutant
- Accumulation of extra chromosome equivalents PubMed
- Defect in biofilm formation, this involves the kinase activity, but the target protein is unknown PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot), autophosphorylation, phosphorylation of Ugd, TuaD, Ssb, SsbB
- Protein family: BY-kinase, see the Bacterial Protein Tyrosine Kinase Database)
- Paralogous protein(s): EpsB
Extended information on the protein
- Kinetic information:
- Domains: single BY-kinase domain
- Modification: autophosphorylation at residues Y225, Y227 and Y228 (primary site) PubMed, dephosphorylated by PtpZ PubMed
- Cofactors: ATP
- Effectors of protein activity: TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation PubMed
Database entries
- Structure: 2VED (CapB, the homolog in Staphylococcus aureus)
- UniProt: P96716
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- KO strain created with pMUTIN-2, available from Ivan Mijakovic
- GP1520 (spc), available in Jörg Stülke's lab
- GP1544 (ermC), available in Jörg Stülke's lab
- GP1587 (cat) , available in Jörg Stülke's lab
- GP1521 epsB (aphA3) ptkA (spc) double mutant available in Jörg Stülke's lab
- GP1529 tkmA-ptkA::spc available in Jörg Stülke's lab
- GP1610 (ptkA-ptpZ, spc), available in Jörg Stülke's lab
- Expression vector: pQE-30, N-terminally 6xHis-tagged, available from Ivan Mijakovic
- lacZ fusion: in a KO strain created with pMUTIN-2, available from Ivan Mijakovic
- GFP fusion: CFP-fusion, available from Ivan Mijakovic
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Ivan Mijakovic, Thiverval-Grignon, France
Your additional remarks
References
Reviews
<pubmed> 20497498 </pubmed>
Original publications
<pubmed> 12970183, 15741737, 15866923, 17367396, 19258708 18547145 20497499 20509597 20815827 20817675 23939619</pubmed>