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| * '''Description:''' 2-methylcitrate synthase <br/><br/>
| | {{#bank: MmgD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mmgD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiO, prpC ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || 2-methylcitrate synthase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24140 mmgD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.202
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1116 bp, 372 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mmgE]]'', ''[[mmgC]]''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mmgD_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mmgD_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mmgD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_2509654_2510772_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mmgD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24140]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[poorly characterized/ putative enzymes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[SigE regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24140
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mmgABCDE-yqiQ.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11322]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Propanoyl-CoA + H<sub>2</sub>O + oxaloacetate = (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA (according to Swiss-Prot)
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| * '''Protein family:''' citrate synthase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[CitA]], [[CitZ]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P45858 P45858]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24140]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.3.5 2.3.3.5]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[mmgA]]-[[mmgB]]-[[mmgC]]-[[mmgD]]-[[mmgE]]-[[yqiQ]]'' {{PubMed|8759838}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_2509654_2510772_-1 mmgD] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigE]] {{PubMed|15699190,8759838}}
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| * '''Regulation:'''
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| ** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|15699190,8759838}}
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| ** repressed by glucose ([[CcpA]]) {{PubMed|8759838}}
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| ** expression is reduced in a [[SigV]] mutant {{PubMed|21926231}}
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| * '''Regulatory mechanism:'''
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| **[[CcpA]]: transcription repression {{PubMed|8759838}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|21926231}}
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| <pubmed>8759838,15699190, </pubmed>
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| [[Category:Protein-coding genes]]
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