Mbl: Difference between revisions
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* '''Description:''' [[cell shape]]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex <br/><br/> | * '''Description:''' [[cell shape]]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for [[CwlO]] activity <br/><br/> | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
* non-viable in the presence of low Mg(2+) | |||
non-viable in the presence of low Mg(2+) | * readily accumulate ''[[rsgI]]'' suppressor mutants [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed] | ||
=== Database entries === | === Database entries === | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
** forms helical filaments in a heterologous system {{PubMed|21091501}} | ** forms helical filaments in a heterologous system {{PubMed|21091501}} | ||
** required for [[CwlO]] activity {{PubMed|23869552}} | |||
* '''Protein family:''' ftsA/mreB family (according to Swiss-Prot) | * '''Protein family:''' ftsA/mreB family (according to Swiss-Prot) | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
* '''Domains:''' | * '''[[Domains]]:''' | ||
* '''Modification:''' | * '''Modification:''' | ||
* ''' | * '''[[Cofactors]]:''' | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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<pubmed> 17064365, 20566861 21636744,21636745 22069484</pubmed> | <pubmed> 17064365, 20566861 21636744,21636745 22069484</pubmed> | ||
==Other original publications== | ==Other original publications== | ||
<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732</pubmed> | <pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732 23869552</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:41, 14 December 2013
- Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for CwlO activity
| Gene name | mbl |
| Synonyms | |
| Essential | no |
| Product | MreB-like protein |
| Function | cell shape determination |
| Gene expression levels in SubtiExpress: mbl | |
| Interactions involving this protein in SubtInteract: Mbl | |
| MW, pI | 35 kDa, 5.669 |
| Gene length, protein length | 999 bp, 333 aa |
| Immediate neighbours | flhO, spoIIID |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Categories containing this gene/protein
cell shape, sporulation proteins, membrane proteins
This gene is a member of the following regulons
SigE regulon, stringent response
The gene
Basic information
- Locus tag: BSU36410
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: ftsA/mreB family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- during logarithmic growth, Mbl forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis PubMed
- forms transverse bands as cells enter the stationary phase PubMed
- close to the inner surface of the cytoplasmic membrane PubMed
- reports on helical structures formed by Mbl PubMed seem to be misinterpretation of data PubMed
Database entries
- Structure:
- UniProt: P39751
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for mbl PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody: available in the Jeff Errington and Peter Graumann labs
Labs working on this gene/protein
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
References
Localization
<pubmed> 17064365, 20566861 21636744,21636745 22069484</pubmed>
Other original publications
<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732 23869552</pubmed>