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| * '''Description:''' formylmethionine deformylase <br/><br/>
| | {{#bank: DefA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''defA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloK, def ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || formylmethionine deformylase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15720 defA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.731
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 480 bp, 160 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[priA]]'', ''[[fmt]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15720 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15720 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15720 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:def_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=defA_1646512_1646994_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:def_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15720]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[protein modification]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU15720
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11933]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Formyl-L-methionyl peptide + H<sub>2</sub>O = formate + methionyl peptide (according to Swiss-Prot)
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| * '''Protein family:''' polypeptide deformylase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[DefB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[DefA]]-[[RplV]] {{PubMed|18288106}}
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| ** [[Map]] competes with peptide deformylase ([[DefA]]), the first enzyme to act on nascent chains, for binding sites at the ribosomal tunnel exit {{PubMed|23770820}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94462 P94462]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15720]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.5.1.88 3.5.1.88]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[defA]]-[[fmt]]-[[yloM]]'' {{PubMed|16964327}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=defA_1646512_1646994_1 defA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] ''[[defA]]-[[fmt]]-[[yloM]]'' {{PubMed|16964327}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>11429456,12627383, 18288106 16964327 23770820</pubmed>
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| [[Category:Protein-coding genes]]
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