DefA

• Description: formylmethionine deformylase
  
  

Gene name	defA
Synonyms	yloK, def
Essential	no
Product	formylmethionine deformylase
Function	translation
Gene expression levels in SubtiExpress: defA
MW, pI	17 kDa, 4.731
Gene length, protein length	480 bp, 160 aa
Immediate neighbours	priA, fmt
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, protein modification

The gene

Basic information

• Locus tag: BSU15720

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Formyl-L-methionyl peptide + H₂O = formate + methionyl peptide (according to Swiss-Prot)

• Protein family: polypeptide deformylase family (according to Swiss-Prot)

• Paralogous protein(s): DefB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DefA-RplV PubMed
  • Map competes with peptide deformylase (DefA), the first enzyme to act on nascent chains, for binding sites at the ribosomal tunnel exit PubMed

• Localization:

Database entries

• Structure:

• UniProt: P94462

• KEGG entry: [2]

• E.C. number: 3.5.1.88

Expression and regulation

• Operon: defA-fmt-yloM PubMed

• Expression browser: defA PubMed

• Sigma factor: SigA defA-fmt-yloM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>11429456,12627383, 18288106 16964327 23770820</pubmed>