Methods: Difference between revisions
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* Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium | * Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium | ||
== | ==Microscopic techniques== | ||
* Live cell imaging of ''B. subtilis'' cells using automated time-lapse microscopy {{PubMed|21841760}} | * Live cell imaging of ''B. subtilis'' cells using automated time-lapse microscopy {{PubMed|21841760}} | ||
* Assay of gene expression dynamics using live cell imaging {{PubMed|22179594}} | * Assay of gene expression dynamics using live cell imaging {{PubMed|22179594}} | ||
* Visualization and quantification of gene expression heterogeneity in growing microbial cells {{PubMed|23874729}} | |||
==Transposon mutagenesis== | ==Transposon mutagenesis== | ||
Revision as of 15:17, 25 July 2013
Here you can find a list of methods for working with Bacillus subtilis:
Media
Analysis of protein-protein interactions
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
Analysis of membrane proteins
- application of a split green fluorescent protein reporter PubMed
Protein expression
- the LIKE system PubMed
Strain construction
- introduction of markerfree deletions: PubMed
RNA analysis
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
Genetic work with non-standard strains
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
- efficient electroporation of B. subtilis PubMed
- a novel transformation protocol for B. subtilis DB104 PubMed
General procedures
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
Microscopic techniques
- Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
- Assay of gene expression dynamics using live cell imaging PubMed
- Visualization and quantification of gene expression heterogeneity in growing microbial cells PubMed
Transposon mutagenesis
- mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed
Protein-DNA interactions
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
Key references
Additional publications: PubMed <pubmed>22179594 20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626</pubmed>