Revision as of 15:03, 16 May 2013

Description: GTP-binding protein, putative tRNA modification GTPase

Gene name	thdF
Synonyms	mnmE
Essential	no
Product	putative tRNA modification GTPase
Function	tRNA modification
*Gene expression levels in SubtiExpress*: thdF
*Interactions involving this protein in SubtInteract*: ThdF
MW, pI	50 kDa, 4.643
Gene length, protein length	1377 bp, 459 aa
Immediate neighbours	gidA, jag
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

translation, GTP-binding proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

Locus tag: BSU41020

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family: MnmE subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:
- GidA-ThdF (suggested by interaction of E. coli counterparts PubMed and by co-localization on the chromosome)

Localization: cytoplasm (according to Swiss-Prot)

Database entries

Structure:

UniProt: P25811

KEGG entry: [3]

E.C. number:

Additional information

Information on the corresponding protein from E. coli: EcoCyc MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

Expression and regulation

Operon: thdF-gidA-rsmG-noc PubMed

Expression browser: thdF PubMed

Sigma factor:

Regulation:

Regulatory mechanism:
- ComK: transcription activation PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Original publications

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