Revision as of 11:09, 14 May 2013

Description: two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)

Gene name	comA
Synonyms	srfB, comAA
Essential	no
Product	two-component system response regulator
Function	regulation of genetic competence and quorum sensing
*Gene expression levels in SubtiExpress*: comA
*Interactions involving this protein in SubtInteract*: ComA
MW, pI	23 kDa, 4.759
Gene length, protein length	642 bp, 214 aa
Immediate neighbours	yuxO, comP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, phosphoproteins

This gene is a member of the following regulons

The ComA regulon

The gene

Basic information

Locus tag: BSU31680

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: ComA family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification: phosphorylated by ComP on an Asp residue

Cofactor(s):

Effectors of protein activity:
- phosphorylation activates DNA binding
- DNA-binding activity of ComA is inhibited by RapC PubMed, RapD PubMed, RapF PubMed, RapH PubMed and RapK PubMed

Interactions:
- RapC-ComA PubMed
- RapF-ComA PubMed
- RapH-ComA PubMed
- ComP-ComA

Localization: cytoplasm (according to Swiss-Prot)

Database entries

Structure:
- 1U83
- 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

UniProt: P14204

KEGG entry: [3]

E.C. number: 4.4.1.19

Additional information

Expression and regulation

Operon: comA-yuxO PubMed

Expression browser: comA PubMed

Sigma factor: SigA PubMed

Regulation:

Regulatory mechanism:

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxO]]'', ''[[comP]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31680 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31680 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:comA_context.gif]]