Revision as of 14:05, 13 May 2013

Description: phosphotransferase of the sporulation initiation phosphorelay

Gene name	spo0F
Synonyms
Essential	no
Product	phosphotransferase of the sporulation initiation phosphorelay
Function	initiation of sporulation
*Gene expression levels in SubtiExpress*: spo0F
*Interactions involving this protein in SubtInteract*: Spo0F
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	14 kDa, 4.697
Gene length, protein length	372 bp, 124 aa
Immediate neighbours	fbaA, ywjG
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

phosphorelay, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The gene

Basic information

Locus tag: BSU37130

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family:

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:
- phosphorylated on an Asp residue by KinA, KinB, KinC, KinD, or KinE
- dephosphorylated by RapA, RapB, RapE, or RapH PubMed

Cofactor(s):

Effectors of protein activity:

Interactions:
- Spo0F-Spo0B PubMed
- Spo0F-Spo0E PubMed
- RapA-Spo0F PubMed
- RapB-Spo0F PubMed
- RapE-Spo0F
- RapH-Spo0F PubMed
- KinA-Spo0F
- KinB-Spo0F
- KinC-Spo0F
- KinD-Spo0F
- KinE-Spo0F
- YmcA-Spo0F PubMed
- RapP-Spo0F, dephosphorylation of Spo0F PubMed
- RapJ-Spo0F, dephosphorylation of Spo0F PubMed

Localization: cytoplasm (according to Swiss-Prot)

Database entries

Structure:
- 1SRR (phosphatase resistant mutant)
- 1F51 (complex with Spo0B)
- 2JVK (Mutant L66A),
- 2FSP
- 3Q15 (RapH-Spo0F complex) PubMed

UniProt: P06628

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: spo0F PubMed

Expression browser: spo0F PubMed

Sigma factor: SigA PubMed, SigH PubMed

Regulation:
- expressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism:
- Spo0A: transcription activation PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37130 spo0F]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37130 spo0F]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Spo0F Spo0F]
@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fbaA]]'', ''[[ywjG]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15730&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37130 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:spo0F_context.gif]]