Revision as of 13:07, 13 May 2013

Description: spore coat protein (inner)

Gene name	cotD
Synonyms
Essential	no
Product	spore coat protein (inner)
Function	resistance of the spore
*Gene expression levels in SubtiExpress*: cotD
MW, pI	8 kDa, 7.373
Gene length, protein length	225 bp, 75 aa
Immediate neighbours	ypsA, yprB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

Locus tag: BSU22200

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization:
- inner spore coat PubMed

Database entries

Structure:

UniProt: P07791

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: cotD (according to DBTBS)

Expression browser: cotD PubMed

Sigma factor: SigK PubMed

Regulation:
- expressed late during sporulation in the mother cell (SigK, SpoIIID) PubMed

Regulatory mechanism:
- SpoIIID: transcription repression PubMed

Additional information:

Biological materials

Mutant:
- 1S104 ( cotD::cat), PubMed, available at BGSC

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || resistance of the spore
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22200 cotD]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22200 cotD]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 7.373
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ypsA]]'', ''[[yprB]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14137&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22200 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22200 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22200 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:cotD_context.gif]]