Tkt: Difference between revisions
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* '''Description:''' transketolase <br/><br/> | |||
{| align="right" border="1" cellpadding="2" | |||
|- | |||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |||
|''tkt'' | |||
|- | |||
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tktA '' | |||
|- | |||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |||
|- | |||
|style="background:#ABCDEF;" align="center"| '''Product''' || transketolase | |||
|- | |||
|style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway | |||
|- | |||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17890 tkt] | |||
|- | |||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Tkt Tkt] | |||
|- | |||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | |||
|- | |||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 4.803 | |||
|- | |||
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2001 bp, 667 aa | |||
|- | |||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzC]]'', ''[[sirA]]'' | |||
|- | |||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13673]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |||
|- | |||
|- | |||
|- | |||
|colspan="2" | '''Genetic context''' <br/> [[Image:tkt_context.gif]] | |||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | |||
|- | |||
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tkt_1919861_1921864_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tkt_expression.png|500px]] | |||
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|} | |||
__TOC__ | |||
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= [[Categories]] containing this gene/protein = | |||
{{SubtiWiki category|[[carbon core metabolism]]}}, | |||
{{SubtiWiki category|[[phosphoproteins]]}} | |||
= This gene is a member of the following [[regulons]] = | |||
{{SubtiWiki regulon|[[Spo0A regulon]]}} | |||
=The gene= | |||
=== Basic information === | |||
* '''Locus tag:''' BSU17890 | |||
===Phenotypes of a mutant === | |||
=== Database entries === | |||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tkt.html] | |||
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11247] | |||
=== Additional information=== | |||
=The protein= | |||
=== Basic information/ Evolution === | |||
* '''Catalyzed reaction/ biological activity:''' Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot) | |||
* '''Protein family:''' transketolase family (according to Swiss-Prot) | |||
* '''Paralogous protein(s):''' | |||
=== Extended information on the protein === | |||
* '''Kinetic information:''' | |||
* '''Domains:''' | |||
* '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed] | |||
* '''Cofactor(s):''' thiamine pyrophosphate | |||
* '''Effectors of protein activity:''' | |||
* '''[[SubtInteract|Interactions]]:''' [[Tkt]]-[[Tpi]] {{PubMed|19193632}} | |||
* '''[[Localization]]:''' | |||
=== Database entries === | |||
* '''Structure:''' [[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3K95 3K95]], complex with thiamine diphosphate, from ''B. anthracis'' | |||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P45694 P45694] | |||
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17890] | |||
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.2.1.1 2.2.1.1] | |||
=== Additional information=== | |||
=Expression and regulation= | |||
* '''Operon:''' ''tkt'' {{PubMed|9068642}} | |||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tkt_1919861_1921864_1 tkt] {{PubMed|22383849}} | |||
* '''Sigma factor:''' | |||
* '''Regulation:''' | |||
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | |||
* '''Regulatory mechanism:''' | |||
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | |||
* '''Additional information:''' | |||
=Biological materials = | |||
* '''Mutant:''' BS4530 (aphA3), available in [[Stülke]] lab | |||
* '''Expression vector:''' | |||
** pGP94 (N-terminal Strep-tag, for [[SPINE]], expression in B. subtilis, in [[pGP380]]), available in [[Stülke]] lab | |||
** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP820, available in [[Stülke]] lab | |||
* '''lacZ fusion:''' | |||
* '''GFP fusion:''' | |||
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | |||
* '''Antibody:''' | |||
* '''FLAG-tag construct:''' GP1406 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab | |||
=Labs working on this gene/protein= | |||
=Your additional remarks= | |||
=References= | |||
==Reviews== | |||
<pubmed>9924800 </pubmed> | |||
==Original publications== | |||
<pubmed>14651647,16493705, 19603213 9068642 16143417 15375635 19193632 </pubmed> | |||
[[Category:Protein-coding genes]] | |||
Revision as of 20:12, 8 August 2012
- Description: transketolase
| Gene name | tkt |
| Synonyms | tktA |
| Essential | no |
| Product | transketolase |
| Function | pentose phosphate pathway |
| Gene expression levels in SubtiExpress: tkt | |
| Interactions involving this protein in SubtInteract: Tkt | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 72 kDa, 4.803 |
| Gene length, protein length | 2001 bp, 667 aa |
| Immediate neighbours | ynzC, sirA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17890
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)
- Protein family: transketolase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylated on ser/ thr/ tyr PubMed
- Cofactor(s): thiamine pyrophosphate
- Effectors of protein activity:
Database entries
- Structure: [3K95], complex with thiamine diphosphate, from B. anthracis
- UniProt: P45694
- KEGG entry: [3]
- E.C. number: 2.2.1.1
Additional information
Expression and regulation
- Operon: tkt PubMed
- Sigma factor:
- Additional information:
Biological materials
- Mutant: BS4530 (aphA3), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
<pubmed>9924800 </pubmed>
Original publications
<pubmed>14651647,16493705, 19603213 9068642 16143417 15375635 19193632 </pubmed>