WalR

• Description: two-component response regulator, controls cell wall metabolism
  
  

Gene name	walR
Synonyms	yycF
Essential	yes PubMed
Product	two-component response regulator
Function	control of cell wall metabolism
Interactions involving this protein in SubtInteract: WalR
MW, pI	27 kDa, 4.876
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	walK, trnY-Phe
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall/ other, transcription factors and their control, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU40410

Phenotypes of a mutant

• essential PubMed
• a constitutively active form of WalR (WalR-R204C) results in constitutive expression of the WalR regulon, and in combination with a sepF mutation to the formation of cell wall-less L-forms PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component transcription regulators

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by WalK on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions:
  • WalK-WalR

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2D1V (DNA binding domain), 2ZWM (receiver domain)

• UniProt: P37478

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-walJ-yyxA PubMed

• Expression browser: walR PubMed

• Sigma factor:

• Regulation: expressed during vegetative growth, repressed during stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Kevin Devine, Dublin

References

Reviews

<pubmed>19019149, 18245295,18792692, 22126136 </pubmed>

The WalR regulon

<pubmed>17581128,10878122,12950927, </pubmed>

Other original Publications

Additional publications: PubMed <pubmed>18573169,17350627,10094672,17827301,9829949,16030236,19574649, 20059685 20111065 20167622 20834167 </pubmed>