Methods: Difference between revisions
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Here you can find a list of methods for working with ''Bacillus subtilis'': | Here you can find a list of methods for working with ''Bacillus subtilis'': | ||
*Bacterial Two Hybrid: [[BACTH]] | ==Analysis of protein-protein interactions== | ||
* Bacterial Two Hybrid: [[BACTH]] | |||
*[[SPINE]]: A method to detect ''in vivo'' [[protein-protein interactions]] | * [[SPINE]]: A method to detect ''in vivo'' [[protein-protein interactions]] | ||
* ''in vivo'' detection of [[protein-protein interactions]] using [[DivIVA]] and GFP {{PubMed|19698693}} | |||
==Strain construction== | |||
* introduction of markerfree deletions: {{PubMed|20057169}} | * introduction of markerfree deletions: {{PubMed|20057169}} | ||
* generation of markerfree mutations: {{PubMed|20134232}} and {{PubMed|21542786}} | * generation of markerfree mutations: {{PubMed|20134232}} and {{PubMed|21542786}} | ||
==RNA analysis== | |||
* Mechanical cell disruption and extraction of RNA from ''B. subtilis'': [[Media:SOP-RNA.pdf]] | * Mechanical cell disruption and extraction of RNA from ''B. subtilis'': [[Media:SOP-RNA.pdf]] | ||
==Work with non-domesticated strains== | |||
* transformation of wild-type ''B. subtilis'' strains incl. NCIB3610: {{PubMed|20300532}} | * transformation of wild-type ''B. subtilis'' strains incl. NCIB3610: {{PubMed|20300532}} | ||
==General procedures== | |||
* Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium | * Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium | ||
* '' | ==Live cell imaging== | ||
* Live cell imaging of ''B. subtilis'' cells using automated time-lapse microscopy {{PubMed|21841760}} | |||
* Assay of gene expression dynamics using live cell imaging {{PubMed|22179594}} | |||
==Transposon mutagenesis== | |||
* mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions {{PubMed|22113911}} | |||
==Protein-DNA interactions== | |||
* ChAP-chip: A modified ChIP-chip protocol for the ''in vivo'' identification of binding sites of DNA-binding proteins {{PubMed|17932079}} | * ChAP-chip: A modified ChIP-chip protocol for the ''in vivo'' identification of binding sites of DNA-binding proteins {{PubMed|17932079}} | ||
==Key references== | ==Key references== | ||
<pubmed>20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 22113911 </pubmed> | <pubmed>20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 22113911 22179594 </pubmed> | ||
Revision as of 16:51, 1 January 2012
Here you can find a list of methods for working with Bacillus subtilis:
Analysis of protein-protein interactions
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
Strain construction
- introduction of markerfree deletions: PubMed
RNA analysis
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
Work with non-domesticated strains
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
General procedures
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
Live cell imaging
- Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
- Assay of gene expression dynamics using live cell imaging PubMed
Transposon mutagenesis
- mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed
Protein-DNA interactions
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
Key references
<pubmed>20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 22113911 22179594 </pubmed>