Methods: Difference between revisions
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* Live cell imaging of B. subtilis cells using automated time-lapse microscopy {{PubMed|21841760}} | * Live cell imaging of B. subtilis cells using automated time-lapse microscopy {{PubMed|21841760}} | ||
* mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions {{PubMed|22113911}} | |||
==Key references== | ==Key references== | ||
<pubmed>20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626</pubmed> | <pubmed>20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 22113911 </pubmed> | ||
Revision as of 11:28, 20 December 2011
Here you can find a list of methods for working with Bacillus subtilis:
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- introduction of markerfree deletions: PubMed
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
- Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
- mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed
Key references
<pubmed>20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 22113911 </pubmed>