SubtInteract: Difference between revisions
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==Methods to detect protein-protein interactions== | ==Methods to detect protein-protein interactions== | ||
* Bacterial Two Hybrid System: [[BACTH]] | * Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}} | ||
* Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}} | * Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}} | ||
* [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking | * [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking {{PubMed|17994626}} | ||
* TAP-Tag purification {{PubMed|10504710,11403571}} | * TAP-Tag purification {{PubMed|10504710,11403571}} | ||
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}} | * ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}} | ||
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|10504710,11403571}} | |||
==Important publications== | ==Important publications== | ||
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 </pubmed> | <pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 </pubmed> | ||
Revision as of 21:15, 3 January 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Finally, many interactions may be of a transient nature.
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Important publications
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 </pubmed>