LevD

• Description: fructose-specific phosphotransferase system, EIIA component
  
  

Gene name	levD
Synonyms	sacL
Essential	no
Product	fructose-specific phosphotransferase system, EIIA component
Function	fructose uptake and phosphorylation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	16 kDa, 4.479
Gene length, protein length	438 bp, 146 aa
Immediate neighbours	levE, levR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27070

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, mannose permease (Man) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P26379

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: levD-levE-levF-levG-sacC

• Sigma factor: SigL

• Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose (LevR)

• Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose

induction: transcription activation by LevR

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>10627040 2117666 1619665 7592487 7592486 9033408, </pubmed>