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* '''Description:''' unknown <br/><br/>
* '''Description:''' assembly/ stability of the 30S subunit of the ribosome, assembly of the 70S ribosome <br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ypuD''
|''yqeH''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|style="background:#ABCDEF;" align="center"| '''Product''' || GTPase
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|style="background:#ABCDEF;" align="center"|'''Function''' || assembly/ stability of the 30S subunit of the ribosome, assembly of the 70S ribosome
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 7.027  
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 6.009  
|-
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 342 bp, 114 aa  
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1098 bp, 366 aa  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ypuE]]'', ''[[sipS]]''
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[aroD]]'', ''[[yqeG]]''
|-
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14262&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14509&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ypuD_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:yqeH_context.gif]]
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|-
Line 35: Line 35:
=== Basic information ===
=== Basic information ===


* '''Locus tag:''' BSU23300
* '''Locus tag:''' BSU25670


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]


=== Database entries ===
=== Database entries ===


* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ypuD.html]
* '''DBTBS entry:''' no entry


* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10516]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11636]


=== Additional information===
=== Additional information===
Line 52: Line 54:
=== Basic information/ Evolution ===
=== Basic information/ Evolution ===


* '''Catalyzed reaction/ biological activity:'''  
* '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP


* '''Protein family:'''
* '''Protein family:''' Era/Obg family


* '''Paralogous protein(s):'''
* '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YphC]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]


=== Extended information on the protein ===
=== Extended information on the protein ===
Line 76: Line 78:
=== Database entries ===
=== Database entries ===


* '''Structure:'''
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EC1 3EC1] (Geobacillus stearothermophilus)


* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P17616 P17616]
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P54453 P54453]


* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23300]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU25670]


* '''E.C. number:'''
* '''E.C. number:'''
Line 90: Line 92:
* '''Operon:'''  
* '''Operon:'''  


* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed],  [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
* '''[[Sigma factor]]:'''  


* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
* '''Regulation:'''  


* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  


* '''Additional information:'''
* '''Additional information:'''  


=Biological materials =
=Biological materials =
Line 113: Line 115:


=Labs working on this gene/protein=
=Labs working on this gene/protein=
[[Naotake Ogasawara]], Nara, Japan


=Your additional remarks=
=Your additional remarks=
Line 118: Line 122:
=References=
=References=


<pubmed>11544224, </pubmed>
<pubmed>12427945, </pubmed>
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
# Morimoto T, Loh PC, Hirai T, Asai K, Kobayashi K, Moriya S, Ogasawara N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in ''Bacillus subtilis''. ''Microbiology'' '''148:''' 3539-3552. [http://www.ncbi.nlm.nih.gov/sites/entrez/12427945 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:49, 8 June 2009

  • Description: assembly/ stability of the 30S subunit of the ribosome, assembly of the 70S ribosome

Gene name yqeH
Synonyms
Essential yes PubMed
Product GTPase
Function assembly/ stability of the 30S subunit of the ribosome, assembly of the 70S ribosome
MW, pI 40 kDa, 6.009
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours aroD, yqeG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU25670

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 3EC1 (Geobacillus stearothermophilus)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

<pubmed>12427945, </pubmed>

  1. Morimoto T, Loh PC, Hirai T, Asai K, Kobayashi K, Moriya S, Ogasawara N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis. Microbiology 148: 3539-3552. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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