Revision as of 11:40, 11 June 2009

Description: major component of biofilm matrix, translocation-dependent antimicrobial spore component

Gene name	tasA
Synonyms	cotN, yqhF
Essential	no
Product	major component of biofilm matrix
Function	biofilm formation
MW, pI	28 kDa, 5.442
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	sinR, sipW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

Locus tag: BSU24620

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: peptidase M73 family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization: extracellular (signal peptide) PubMed, membrane PubMed

Database entries

Structure:

Swiss prot entry:

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: yqxM-sipW-tasA PubMed

Sigma factor: SigA PubMed

Regulation: repressed by SinR PubMed, repressed by casamino acids PubMed
- repressed during logrithmic growth (AbrB) PubMed

Regulatory mechanism: SinR: transcription repression PubMed
- AbrB: transcription repression PubMed
- AbrB: transcription repression PubMed
- AbrB: transcription repression PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed

@@ Line 1: / Line 1: @@
-#REDIRECT [[SdpB]]
+* '''Description:''' major component of biofilm matrix, translocation-dependent antimicrobial spore component <br/><br/>
+{| align="right" border="1" cellpadding="2"
+|-
+|style="background:#ABCDEF;" align="center"|'''Gene name'''
+|''tasA''
+|-
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''cotN, yqhF ''
+|-
+|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+|-
+|style="background:#ABCDEF;" align="center"| '''Product''' || major component of biofilm matrix
+|-
+|style="background:#ABCDEF;" align="center"|'''Function''' || biofilm formation
+|-
+|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.442
+|-
+|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 783 bp, 261 aa
+|-
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sinR]]'', ''[[sipW]]''
+|-
+|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14393&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|-
+|colspan="2" | '''Genetic context''' <br/> [[Image:tasA_context.gif]]
+ <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
+|-
+|}
+__TOC__
+<br/><br/>
+=The gene=
+=== Basic information ===
+* '''Locus tag:''' BSU24620
+===Phenotypes of a mutant ===
+=== Database entries ===
+* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqxM-sipW-tasA.html]
+* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11697]
+=== Additional information===
+=The protein=
+=== Basic information/ Evolution ===
+* '''Catalyzed reaction/ biological activity:'''
+* '''Protein family:''' peptidase M73 family (according to Swiss-Prot)
+* '''Paralogous protein(s):'''
+=== Extended information on the protein ===
+* '''Kinetic information:'''
+* '''Domains:'''
+* '''Modification:'''
+* '''Cofactor(s):'''
+* '''Effectors of protein activity:'''
+* '''Interactions:'''
+* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed],  membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
+=== Database entries ===
+* '''Structure:'''
+* '''Swiss prot entry:'''
+* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24620]
+* '''E.C. number:'''
+=== Additional information===
+=Expression and regulation=
+* '''Operon:''' ''[[yqxM]]-[[sipW]]-[[tasA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed]
+* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed]
+* '''Regulation:''' repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed], repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
+** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]
+* '''Regulatory mechanism:''' [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed]
+** [[AbrB]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]
+** [[AbrB]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]
+** [[AbrB]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]
+* '''Additional information:'''
+=Biological materials =
+* '''Mutant:'''
+* '''Expression vector:'''
+* '''lacZ fusion:'''
+* '''GFP fusion:'''
+* '''two-hybrid system:'''
+* '''Antibody:'''
+=Labs working on this gene/protein=
+=Your additional remarks=
+=References=
+<pubmed>18957862 12107147 18763711, </pubmed>
+# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
+# M&#228;der et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
+# Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics '''8:''' 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
+# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]