Revision as of 11:46, 11 June 2009

Description: repressor of the arabinose utilization genes

Gene name	araR
Synonyms	araC, yvbS
Essential	no
Product	transcriptional repressor (LacI family))
Function	regulation of arabinose utilization (araABDLMNPQ-abfA) and of the araE/araR genes
MW, pI	43 kDa, 6.341
Gene length, protein length	1152 bp, 384 aa
Immediate neighbours	araE, yvbT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

Locus tag: BSU33970

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family:

Paralogous protein(s):

Genes/ operons controlled by AraR

Activated by AraR:
Repressed by AraR: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization: cytoplasm (according to Swiss-Prot)

Database entries

Structure:

Swiss prot entry: P96711

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon:

Sigma factor:

Regulation:
- repressed in the absence of arabinose (AraR) PubMed

Regulatory mechanism:
- AraR: transcription repression PubMed
- AraR: transcription repression PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mota LJ, Tavares P, Sá-Nogueira I. (1999) Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis. Mol Microbiol. Aug;33(3): 476-89. PubMed
Sá-Nogueira I, Mota LJ. (1997) Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. J Bacteriol. Mar;179(5): 1598-608. PubMed

@@ Line 1: / Line 1: @@
-#REDIRECT [[Abf2]]
+* '''Description:''' repressor of the arabinose utilization genes <br/><br/>
+{| align="right" border="1" cellpadding="2"
+|-
+|style="background:#ABCDEF;" align="center"|'''Gene name'''
+|''araR''
+|-
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''araC, yvbS ''
+|-
+|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+|-
+|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor (LacI family))
+|-
+|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of arabinose utilization
+(araABDLMNPQ-abfA) and of the araE/araR genes
+|-
+|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 6.341
+|-
+|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1152 bp, 384 aa
+|-
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[araE]]'', ''[[yvbT]]''
+|-
+|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15402&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|-
+|colspan="2" | '''Genetic context''' <br/> [[Image:araR_context.gif]]
+ <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
+|-
+|}
+__TOC__
+<br/><br/>
+=The gene=
+=== Basic information ===
+* '''Locus tag:''' BSU33970
+===Phenotypes of a mutant ===
+=== Database entries ===
+* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/araR.html]
+* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11913]
+=== Additional information===
+=The protein=
+=== Basic information/ Evolution ===
+* '''Catalyzed reaction/ biological activity:'''
+* '''Protein family:'''
+* '''Paralogous protein(s):'''
+=== Genes/ operons controlled by AraR ===
+* '''Activated by AraR:'''
+* ''' Repressed by AraR:''' ''[[araA]]-[[araB]]-[[araD]]-[[araL]]-[[araM]]-[[araN]]-[[araP]]-[[araQ]]-[[abfA]]''
+=== Extended information on the protein ===
+* '''Kinetic information:'''
+* '''Domains:'''
+* '''Modification:'''
+* '''Cofactor(s):'''
+* '''Effectors of protein activity:'''
+* '''Interactions:'''
+* '''Localization:''' cytoplasm (according to Swiss-Prot)
+=== Database entries ===
+* '''Structure:'''
+* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P96711 P96711]
+* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU33970]
+* '''E.C. number:'''
+=== Additional information===
+=Expression and regulation=
+* '''Operon:'''
+* '''[[Sigma factor]]:'''
+* '''Regulation:'''
+** repressed in the absence of arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
+* '''Regulatory mechanism:'''
+** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14973026 PubMed]
+** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
+* '''Additional information:'''
+=Biological materials =
+* '''Mutant:'''
+* '''Expression vector:'''
+* '''lacZ fusion:'''
+* '''GFP fusion:'''
+* '''two-hybrid system:'''
+* '''Antibody:'''
+=Labs working on this gene/protein=
+=Your additional remarks=
+=References=
+<pubmed>10417639 9045819, </pubmed>
+# Mota LJ, Tavares P, Sá-Nogueira I. (1999) Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis. ''Mol Microbiol.'' '''Aug;33(3):''' 476-89. [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
+# Sá-Nogueira I, Mota LJ. (1997) Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. ''J Bacteriol. '' '''Mar;179(5):''' 1598-608. [http://www.ncbi.nlm.nih.gov/sites/entrez/9045819 PubMed]