PAC6: Difference between revisions

PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

Revision as of 18:35, 7 September 2009

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenocol resistance

similar vectors: pAC5, pAC7

sequencing primer:

@@ Line 18: / Line 18: @@
 *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
+<pubmed> 11902727 </pubmed>
-Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the ''Bacillus subtilis ptsGHI'' operon by glucose is controlled by a novel antiterminator, ''GlcT Mol Microbiol.''  '''25:''' 65-78. [http://www.ncbi.nlm.nih.gov/sites/entrez/11902727 PubMed]