PAC5: Difference between revisions

PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

Revision as of 18:34, 7 September 2009

pAC5 cloning region: click to enlarge

Vector for the construction of translational lacZ fusions that can be integrated at the amyE site

Does not replicate in B. subtilis, integrates into the amyE site

Constructed in the Rapoport lab

sequencing primers:

@@ Line 2: / Line 2: @@
 [[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']]
-vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site
+Vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site
-does not replicate in ''B. subtilis'', integrates into the ''amyE'' site
+Does not replicate in ''B. subtilis'', integrates into the ''amyE'' site
-constructed in the Rapoport lab
+Constructed in the Rapoport lab
-in ''E. coli'': ampicillin resistance
+* in ''E. coli'': ampicillin resistance
-in ''B. subtilis'': chloramphenicol resistance
+* in ''B. subtilis'': chloramphenicol resistance
-similar vectors: [[pAC6]], [[pAC7]]
+* similar vectors: [[pAC6]], [[pAC7]]
-sequencing primer:
+sequencing primers:
 *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’
 *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘
 *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
+<pubmed> 1619665 </pubmed>
-Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/1619665  PubMed]