PAC6: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] | [[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] | ||
vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
Revision as of 11:32, 16 April 2009
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenocol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT Mol Microbiol. 25: 65-78. PubMed