PWH844: Difference between revisions
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vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein | vector for the expression of proteins in ''E. coli'', the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein | ||
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | the plasmid carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
constructed in the [[Hillen]] lab | constructed in the [[Hillen]] lab | ||
in E. coli: ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
similar plasmid: [[pGP570]] | similar [[plasmids|plasmid]]: [[pGP570]] | ||
sequencing primer: | sequencing primer: | ||
Revision as of 11:29, 16 April 2009
pWH844
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- pWH844 fw: 5’-TATGAGAGGATCGCATCACCAT-3‘
Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed