PAC7: Difference between revisions
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constructed in the Rapoport lab | constructed in the Rapoport lab | ||
in E. coli: ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
in B. subtilis: kanamycin resistance | in ''B. subtilis'': kanamycin resistance | ||
similar vectors: [[pAC5]], [[pAC6]] | similar vectors: [[pAC5]], [[pAC6]] | ||
Revision as of 11:21, 16 April 2009
pAC7
vector for the construction of translational lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: kanamycin resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
Weinrauch et al. (1991) J Bacteriol. 173:5685-5693. PubMed