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| * '''Description:''' mediator of [[RecA]] binding to ssDNA, required for the formation of [[RecA]] DNA repair centers, , required for efficient survival and replication restart after replication-transcription conflicts <br/><br/>
| | {{#bank: RecO}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''recO''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqxN, yqfI ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || mediator of [[RecA]] binding to ssDNA
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25280 recO]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RecO RecO]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 8.208
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyQ]]'', ''[[yqzL]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25280 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25280 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25280 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:recO_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recO_2608946_2609713_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:recO_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25280]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU25280
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| ===Phenotypes of a mutant ===
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| * drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) {{PubMed|24285298}}
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| * ''[[recO]] [[recJ]]'' double mutants are extremely sensitive against DNA damaging agents {{PubMed|26001966}}
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| * ''[[recO]] [[addA]]-[[addB]]'' double mutants are extremely sensitive against DNA damaging agents {{PubMed|26001966}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25280&redirect=T BSU25280]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11189]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** provides [[RecA]] access to ssDNA during chromosomal transformation (together with [[DprA]]) {{PubMed|22373918}}
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| ** catalyzes annealing of [[SsbA]] or [[SsbA]]/[[SsbB]] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation {{PubMed|22373918}}
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| ** required for the formation of [[RecA]] DNA repair centers (together with [[RecR]]) {{PubMed|24891441}}
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| * '''Protein family:''' recO family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SsbA]] (bound to ssDNA) -[[RecO]], this helps to dislodge [[SsbA]] and [[SsbB]] from ssDNA {{PubMed|21170359,22373918}}
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| ** [[RecR]]-[[RecO]], [[RecR]]:[[RecO]] molecular ratio of 2:1 (as judged from other bacteria such as ''E. coli'' and ''Deinococcus radiodurans'') {{PubMed|17581636}}
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| * '''[[Localization]]:'''
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| ** cytoplasm (according to Swiss-Prot)
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| ** localizes to the DNA entry pole during transformation {{PubMed|22373918}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25280&redirect=T BSU25280]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=2V1C 2V1C] (the [[RecR]]-[[RecO]] complex from ''Deinococcus radiodurans'', [[RecR]]: 52% identity, 78% similarity, [[RecO]]: 30%/ 58%) {{PubMed|17581636}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P42095 P42095]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25280]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recO_2608946_2609713_-1 recO] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A892 ( ''recO''::''cat''), {{PubMed|10323239}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 22933559 </pubmed>
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| == Original publications ==
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| <pubmed>24891441,18599486, 19730681 20581116 22373918 17581636 21170359 24285298 24285298 25939832 26001966 15186413</pubmed>
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| [[Category:Protein-coding genes]]
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