|
|
| Line 1: |
Line 1: |
| * '''Description:''' [[two-component systems|two-component]] sensor kinase, phosphorylates [[Spo0F]], part of the [[phosphorelay]], senses changes in respiratory activity <br/><br/>
| | {{#bank: KinB}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''kinB''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] sensor kinase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31450 kinB]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KinB KinB]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=kinB kinB]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 6.682
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1287 bp, 429 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[patB]]'', ''[[kapB]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31450 DNA_with_flanks]
| |
| |-
| |
| |-
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:kinB_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=kinB_3230067_3231353_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:kinB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31450]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[protein modification]]}},
| |
| {{SubtiWiki category|[[transcription factors and their control]]}},
| |
| {{SubtiWiki category|[[phosphorelay]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}},
| |
| {{SubtiWiki category|[[phosphoproteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CodY regulon]]}},
| |
| {{SubtiWiki regulon|[[stringent response]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU31450
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31450&redirect=T BSU31450]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/kinB-kapB.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10745]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
| ** autophosphorylation, phosphorylation of [[Spo0F]]
| |
| ** mainly active in the older, inner regions of a colony (with [[KinA]]) {{PubMed|21097618}}
| |
| ** sensing potassium level in the medium during initiation of [[sliding]] [[sliding]] {{PubMed|26152584}}
| |
|
| |
| * '''Protein family:'''
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
| ** six transmembrane segments, C-terminal histidine phosphotransferase domain
| |
| ** selectivity filter sequence of potassium channels that is required for [[sliding]] but not for [[sporulation]] {{PubMed|26152584}}
| |
|
| |
| * '''Modification:''' autophosphorylation on a His residue
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
| ** activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus {{PubMed|23599347}}
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[KinB]]-[[Sda]]
| |
| ** [[KinB]]-[[Spo0F]]
| |
| ** [[KinB]]-[[CtaC]] {{PubMed|23599347}}
| |
| ** [[KinB]]-[[QcrC]] {{PubMed|23599347}}
| |
|
| |
| * '''[[Localization]]:''' cell membrane (integral membrane protein) {{PubMed|23599347}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31450&redirect=T BSU31450]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3D36 3D36] (from G. stearothermophilus, complex with [[Sda]])
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q08430 Q08430]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31450]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[kinB]]-[[kapB]]'' {{PubMed|8497199}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=kinB_3230067_3231353_1 kinB] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8497199}}
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
| |
| ** induced upon addition of decoyinine (positive [[stringent response]]) {{PubMed|23378509}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
| ** JH19980 (''kinB''::''tet'') {{PubMed|9334321}}
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>26152584,8576055,16166384,9299348,8497199,10094672,9426145,11069677,12618455,11902725,12618455,7592498, 21097618 19101565 23378509 23599347 26152584</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
