SinR

• Description: transcriptional regulator (Xre family) of post-exponential-phase responses genes
  
  

Gene name	sinR
Synonyms	sin, flaD
Essential	no
Product	transcriptional regulator (Xre family) of post-exponential-phase responses genes
Function	control of biofilm formation
Gene expression levels in SubtiExpress: sinR
Interactions involving this protein in SubtInteract: SinR
Metabolic function and regulation of this protein in SubtiPathways: sinR
MW, pI	12 kDa, 7.177
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	sinI, tasA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU24610

Phenotypes of a mutant

• the mutation suppresses the galactose toxicity to a galE mutant PubMed

Database entries

• BsubCyc: BSU24610

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • transcription regulator of biofilm genes, acts as a true repressor of the tapA-sipW-tasA operon and as an anti-activator (prevents binding of the activator protein RemA) of the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon PubMed
  • acts as co-repressor for SlrR PubMed

• Protein family:Xre family

• Paralogous protein(s): SlrR

Extended information on the protein

• Kinetic information:

• Domains:
  • DNA-binding N-terminal domain (aa 1-69) PubMed
  • SinI-binding C-terminal domain (aa 74-111) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • SinI and SlrA are antagonists to SinR PubMed
  • SlrR is also antagonist of SinR PubMed

• Interactions:
  • SlrA-SinR PubMed, K(D) 10.6 nM PubMed
  • SlrR-SinR PubMed, K(D) 47.5 nM PubMed, Veg may inhibit this interaction PubMed
  • SinR-SinI PubMed, K(D) 1.8 nM PubMed
  • SinR-ScoC

• Localization:

Database entries

• BsubCyc: BSU24610

• Structure:
  • 3QQ6 (N-terminal domain, aa 1-69) PubMed
  • 2YAL (C-terminal domain, aa 74-111) PubMed
  • 1B0N (complex SinR-SinI) PubMed

• UniProt: P06533

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • sinI-sinR PubMed
  • sinR PubMed

• Expression browser: sinR PubMed

• Sigma factor:
  • sinI: SigA PubMed
  • sinR: SigA PubMed

• Regulation:
  • sinI: repressed under conditions that trigger sporulation (Spo0A) PubMed
  • sinI: repressed during exponential growth (ScoC) PubMed
  • sinI: repressed during logrithmic growth (AbrB) PubMed
  • sinI: repressed during vegetative growth (SinR) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • SinR: transcription repression PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3.5 to 13 min) PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 699 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 425 PubMed

Biological materials

• Mutant:
  • GP923 (sinR::spec) PubMed, available in Jörg Stülke's lab
  • GP736 (sinR::tetR) PubMed, available in Jörg Stülke's lab
  • 1S97 (sinR::phleo), PubMed, available at BGSC
  • GP1672 (sinR-tasA::cat) PubMed, available in Jörg Stülke's lab
  • GP1663 (yqhG-sinI-sinR-tasA), available in Jörg Stülke's lab

• Expression vector:
  • N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380: pGP1083 , available in Jörg Stülke's lab

• lacZ fusion:
  • pGP1930 (aphA3) based on pAC7, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct: GP960 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

References

Reviews

<pubmed>20395361 20541494 23353768 24988880 </pubmed>

Modelling of the SinI/SinR switch

<pubmed> 21095906 </pubmed>

Original publications

<pubmed> 22893383 23378512 23430750 23475644 21856853 21815947 22329926,21326214,21708175 8955328, 15661000, 8878039, 24317403 16923912,15104138, 16430695,16430696,18047568, 18430133,11751836,1906467, 11751836, 7635837, 11751836, 19201793, 10547280, 15104138, 9799632 19788541 19898538 3125149 8932324 20351052 20923420 8422983 9685500 9158733 23646920 23660663 24256735 24347549 25433524 </pubmed>