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| * '''Description:''' oligopeptide [[ABC transporter]] (binding protein) <br/><br/>
| | {{#bank: OppA}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''oppA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0KA ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || oligopeptide [[ABC transporter]] (binding protein)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation, competence development
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11430 oppA]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=OppA OppA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=OppA OppA]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 61 kDa, 5.722
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1635 bp, 545 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trpS]]'', ''[[oppB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11430 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:oppA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=oppA_1219849_1221486_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:oppA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11430]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ABC transporters]]}},
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| {{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}},
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[ScoC regulon]]}},
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| {{SubtiWiki regulon|[[TnrA regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU11430
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11430&redirect=T BSU11430]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/oppABCDF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10771]
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|
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| === Additional information===
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|
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** required for the uptake of quorum sensing peptides such as [[PhrH]] {{PubMed|21908671}}
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| * '''Protein family:''' bacterial solute-binding protein 5 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[DppE]]
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:''' phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** ([[OppD]]-[[OppF]])-([[OppB]]-[[OppC]])-[[OppA]] {{PubMed|10092453}}
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| ** [[FloT]]-[[OppA]] {{PubMed|23651456}}
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| * '''[[Localization]]:'''
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| ** attached to the cell membrane (via [[OppB]]-[[OppC]]) {{PubMed|10092453}}
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| ** at the outer side of the cell: extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed], lipid modification as retention signal [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11430&redirect=T BSU11430]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P24141 P24141]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11430]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[oppA]]-[[oppB]]-[[oppC]]-[[oppD]]-[[oppF]]'' {{PubMed|1901616}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=oppA_1219849_1221486_1 oppA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12823818 PubMed]
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| ** repressed during exponential growth ([[ScoC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10383984 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12823818 PubMed]
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| ** [[ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10383984 PubMed]
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| ** [[CodY]]: transcription repression, no derepression occurs, however, in a ''[[codY]]'' mutant, due to increased repression by [[ScoC]] {{PubMed|25966844}}
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| * '''Additional information:'''
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| ** A [[ncRNA]] is predicted between ''[[trpS]]'' and ''[[oppA]]'' {{PubMed|20525796}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1937 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 20139 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** BP67 (spc) available in [[Fabian Commichau]]'s lab
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| ** 1S118 ( ''oppA''::''spec''), {{PubMed|11267663}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S118&Search=1S118 BGSC]
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| ** GP2097 (D(''[[oppA]]-[[oppB]]-[[oppC]]-[[oppD]]-[[oppF]]'')::''aphA3''), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>1901616,10092453,12823818,11267663,9252573,10960106,,12823818,10383984,18957862 18763711 17218307, 7997159 20525796 23651456 21908671 25966844 1899858 23199363</pubmed>
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| [[Category:Protein-coding genes]]
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