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| * '''Description:''' [[RNA polymerase]] [[sigma factor]] SigD <br/><br/>
| | {{#bank: SigD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sigD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''flaB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[RNA polymerase]] [[sigma factor]] SigD
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of flagella, motility, chemotaxis and autolysis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16470 sigD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SigD SigD]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.167
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 762 bp, 254 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheD]]'', ''[[swrB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16470 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sigD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigD_1716493_1717257_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sigD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16470]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription]]}},
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| {{SubtiWiki category|[[sigma factors and their control]]}},
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| {{SubtiWiki category|[[motility and chemotaxis]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[DegU regulon]]}},
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| {{SubtiWiki regulon|[[SigD regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The [[SigD regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16470
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| ===Phenotypes of a mutant ===
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| * Inactivation of ''[[alsR]]'', ''[[sigD]]'' and ''[[sigW]]'' increases competitive fitness of ''Bacillus subtilis'' under non-sporulating conditions {{PubMed|22344650}}
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| * mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of ''[[motA]]-[[motB]]'' expression) {{PubMed|24296669}}
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| * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16470&redirect=T BSU16470]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10751]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' sigma-70 factor family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' interaction with [[FlgM]] inhibits SigD activity {{PubMed|10207036}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SigD]]-[[FlgM]] {{PubMed|10207036}}, [[SigD]]-([[RpoB]]-[[RpoC]])
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16470&redirect=T BSU16470]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P10726 P10726]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16470]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' part of the [[fla-che operon]]
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| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
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| ** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
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| ** ''[[sigD]]-[[swrB]]'' {{PubMed|9335309}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigD_1716493_1717257_1 sigD] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
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| ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
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| ** ''[[sigD]]'': [[SigA]] {{PubMed|9335309}}
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| * '''Regulation:''' see [[fla-che operon]]
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| * '''Regulatory mechanism:''' see [[fla-che operon]]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A716 ( ''sigD''::''cat''), {{PubMed|2832368}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A716&Search=1A716 BGSC]
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| ** DS6420 (marker-less in NCIB3610) {{PubMed|22329926}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''FLAG-tag construct:''' GP948 (ermC, based on [[pGP1087]]), available in the [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20233302 25251856 </pubmed>
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| ==The [[SigD regulon]]==
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| <pubmed>15033535,2111808,</pubmed>
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| ==Original publications==
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| <pubmed> 26122431,22344650 22329926,21097624,22956758 21856853 8955328,7708689,9648743, 8169223,9096206,15175317, 2498284,16357223, 3127378, 26170408 9144176,18820022,7602586,2832368,8195064,11987133,,14651647, 9335309 9657996,8157612,15175317 10809682 8932324 20233303 17850253 10207036 16428420 24296669 25099370 24386445</pubmed>
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| [[Category:Protein-coding genes]]
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