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| * '''Description:''' bacterial hydrophobin, forms water-repellent surface layer of the biofilm, inhibitor of [[KinA]] autophosphorylation, and subsequently of entry into [[sporulation]] <br/><br/>
| | {{#bank: BslA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''bslA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yuaB, sivB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || biofilm surface layer, inhibitor of [[KinA]] autophosphorylation
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]], control of entry into [[sporulation]] via the [[phosphorelay]]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31080/ bslA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 9.987
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 543 bp, 181 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gbsR]]'', ''[[ktrA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31080 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31080 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31080 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yuaB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaB_3187503_3188048_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:yuaB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31080]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biofilm formation]]}},
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| {{SubtiWiki category|[[phosphorelay]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[DegU regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU31080
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| ===Phenotypes of a mutant ===
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| * reduced colony complexity {{PubMed|21742882}}
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| * loss of surface repellency of the biofilm {{PubMed|22571672}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31080&redirect=T BSU31080]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12341]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** inhibits [[KinA]] autophophorylation {{PubMed|23335417}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[SivA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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| ** biofilm matrix in an extracellular polysaccharide-dependent manner {{PubMed|22571672}}
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| ** forms a layer on the biofilm surface {{PubMed|22571672}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31080&redirect=T BSU31080]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=4BHU 4BHU] {{PubMed|23904481}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P71014 P71014]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31080]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[bslA]]'' {{PubMed|22383849}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaB_3187503_3188048_1 yuaB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[AbrB]]: transcription repression {{PubMed|18978066}}
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| ** [[LutR]]: transcription activation {{PubMed|24196425}}
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| ** expression is down-regulated in a ''[[rok]]'' mutant (indirect effect) {{PubMed|21097620}}
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| ** [[DegU]]-P: transcription activation {{PubMed|18978066}}, this might be indirect {{PubMed|21742882}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** ''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) {{PubMed|17850253}}
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| ** ''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) {{PubMed|18978066}}
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| ** ''B. subtilis'' ''bslA''::Cm (168 based) {{PubMed|21097620}}
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| * '''Expression vector:'''
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| ** Physpank-bslA based on vector pDR111: pDRyuaB2 {{PubMed|21097620}}
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| * '''lacZ fusion:'''
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| ** pNW500 P''bslA-lacZ'' fusion in pDG1663 {{PubMed|18978066}}
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| * '''GFP fusion:'''
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| ** P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 22607588 23979433 24988880 25907113</pubmed>
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| ==Original publications==
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| <pubmed> 22571672 18978066 18957862 17850253 21097620 21742882 22383849 23335417 23904481 22571672 25870300 26152584</pubmed>
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| [[Category:Protein-coding genes]]
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