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| * '''Description:''' H+-coupled [[MotA]]-[[MotB]] flagellar stator <br/><br/>
| | {{#bank: MotA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''motA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''mot ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || motility protein (flagellar motor rotation)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13690 motA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MotA MotA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.01
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 810 bp, 270 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[motB]]'', ''[[clpE]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13690 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:motA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motA_1434433_1435245_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:motA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13690]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[motility and chemotaxis]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigD regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU13690
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| ===Phenotypes of a mutant ===
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| * loss of swimming and swarming motility {{PubMed|24296669}}
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| * mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
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| * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13690&redirect=T BSU13690]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/motAB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10688]
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| === Additional information===
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| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' motA family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[MotP]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** the interaction with [[DgrA]] inhibits [[MotA]] activity {{PubMed|22821967}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[MotA]]-[[MotB]]
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| ** [[DgrA]]-[[MotA]] {{PubMed|22821967}}
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13690&redirect=T BSU13690]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P28611 P28611]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13690]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[motA]]-[[motB]]'' {{PubMed|6313226}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motA_1434433_1435245_-1 motA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigD]] {{PubMed|6313226}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 100 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 613 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A631 ( ''motA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC]
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| ** 1A923 ( ''motA''::''erm''), {{PubMed|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC]
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| ** DS7498 (marker-less in NCIB3610) {{PubMed|24296669}}
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| ** TB193 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
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| ** TB210 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 26122431, 6313226 21821766 22821967 24296669 24771657 </pubmed>
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| [[Category:Protein-coding genes]]
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