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| * '''Description:''' flagellar basal-body M-ring protein, membrane anchor of the basal body <br/><br/>
| | {{#bank: FliF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''fliF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || flagellar basal-body M-ring protein
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16210 fliF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FliF FliF]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 4.37
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1608 bp, 536 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fliE]]'', ''[[fliG]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16210 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16210 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16210 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:fliF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliF_1692496_1694106_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:fliF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16210]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[motility and chemotaxis]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[DegU regulon]]}},
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| {{SubtiWiki regulon|[[SigD regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16210
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| ===Phenotypes of a mutant ===
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| * mucoid phenotype due to the overproduction of poly-gamma-glutamate {{PubMed|24296669}}
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| * no secretion of [[FlgM]], permanent inhibition of [[SigD]] {{PubMed|25313396}}
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| * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16210&redirect=T BSU16210]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10240]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** required for correct localization of [[FliM]] {{PubMed|23190039}}
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| * '''Protein family:''' fliF family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[FliF]]-[[FliG]] {{PubMed|25313396}}
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| * '''[[Localization]]:'''
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| ** cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16210&redirect=T BSU16210]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P23447 P23447]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16210]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' part of the [[fla-che operon]] (see there for regulation)
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| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliF_1692496_1694106_1 fliF] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
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| * '''Regulation:''' see [[fla-che operon]]
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| * '''Regulatory mechanism:''' see [[fla-che operon]]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 68 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 595 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** DS7080 (marker-less in NCIB3610) {{PubMed|24296669}}
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| ** TB192 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
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| ** TB208 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 26122431, 1905667,17850253,14651647,18763711, 9657996,8157612,15175317 23190039 24296669 25313396 24386445 25843804</pubmed>
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| [[Category:Protein-coding genes]]
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