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| * '''Description:''' competence transcription factor (CTF) <br/><br/>
| | {{#bank: ComK}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''comK''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || competence transcription factor (CTF)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of competence and DNA uptake
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10420 comK]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ComK ComK]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=comK comK]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 7.669
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 576 bp, 192 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhzC]]'', ''[[yhxD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10420 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:comK_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comK_1117109_1117687_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:comK_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10420]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[ComK regulon]]}},
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| {{SubtiWiki regulon|[[DegU regulon]]}},
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| {{SubtiWiki regulon|[[Rok regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The [[ComK regulon]]=
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| =The gene=
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|
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| === Basic information ===
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| * '''Locus tag:''' BSU10420
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|
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| ===Phenotypes of a mutant ===
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| * no expression of competence genes, loss of [[genetic competence]] {{PubMed|7783616}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10420&redirect=T BSU10420]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comK.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11059]
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|
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| === Additional information===
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|
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| =The protein=
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|
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| ** phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 {{PubMed|22517742}}
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[MecA]]-[[ComK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10447896 PubMed]
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| * '''[[Localization]]:'''
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10420&redirect=T BSU10420]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P40396 P40396]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10420]
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| * '''E.C. number:'''
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|
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''comK'' {{PubMed|8196543}}
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|
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comK_1117109_1117687_1 comK] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8196543}}
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| * '''Regulation:'''
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| ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| ** repressed by [[Rok]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8830686 PubMed]
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| ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12586407 PubMed]
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| ** induced at the entry to stationary phase ([[Spo0A]]-P) {{PubMed|22412392}}
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| * '''Regulatory mechanism:'''
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| ** [[Rok]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11849533 PubMed]
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8830686 PubMed]
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| ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12586407 PubMed]
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| ** [[ComK]]: transcription activation (autoregulation) {{PubMed|8083168}}
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| ** [[DegU]] (non-phosphorylated form): transcription activation {{PubMed|8083168}}
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| ** [[Spo0A]]-P: transcription activation, acts as antagonist of [[Rok]] {{PubMed|22412392}}
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| * '''Additional information:'''
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| ** degraded by [[ClpC]]-[[ClpP]] (with [[MecA]] as adaptor protein) {{PubMed|9890793}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 49 {{PubMed|24696501}}
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|
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A871 (no resistance), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]
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| * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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|
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| =Labs working on this gene/protein=
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| [[Oscar Kuipers]], University of Groningen, The Netherlands
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| [http://molgen.biol.rug.nl/molgen/index.php Homepage]
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|
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19995980 12576575 </pubmed>
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| ==The [[ComK regulon]]==
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| <pubmed>11948146,11918817,</pubmed>
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| ==Original Publications==
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| <pubmed>8878039,11849533,17569828,9335269,8016067,17493123,17560370,12586407,11814663,9108277,9890793,9585513,15819619,10908654,9000055,7783616,7746142,8830686,12586407, 10447896, 12028382, 8016067, 10361283, 11703662, 12107147, 17581123, 8016066 17493123,16391071,16436435 12270822 15598897 20300532 12761164 12036071 21085679 22517742 7783616 22412392 8083168 24838881 8196543 26110430</pubmed>
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| [[Category:Protein-coding genes]]
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