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| * '''Description:''' DNA integrity scanning protein,diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests [[DNA replication]] during ''B. subtilis'' spore outgrowth until the germinating spore's genome is free of damage <br/><br/>
| | {{#bank: DisA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''disA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yacK''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || DNA integrity scanning protein, has diadenylate cyclase activity
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[sporulation]] initiation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00880 disA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DisA DisA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=disA disA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.569
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1080 bp, 360 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[radA]]'', ''[[yacL]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00880 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00880 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00880 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yacK_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=disA_107476_108558_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:disA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00880]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell division]]}},
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| {{SubtiWiki category|[[metabolism of signalling nucleotides]]}},
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| {{SubtiWiki category|[[sporulation/ other]]}},
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| {{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
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| {{SubtiWiki category|[[heat shock proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CtsR regulon]]}},
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| {{SubtiWiki regulon|[[SigB regulon]]}},
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| {{SubtiWiki regulon|[[SigM regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU00880
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00880&redirect=T BSU00880]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10150]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** synthesis of c-di-AMP from two molecules of ATP {{PubMed|23192352,21566650}}
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| * '''Protein family:''' disA family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** contains a [[DAC domain]] for the synthesis of c-di-AMP {{PubMed|18439896}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** [[RadA]] is an inhibitor of [[DisA]] activity {{PubMed|23760274}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms octamers
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| ** [[RacA]]-[[DisA]] {{PubMed|12493822}}
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| ** [[RadA]]-[[DisA]] {{PubMed|23760274}}, this interaction result in the inhibition of [[DisA]] activity {{PubMed|23760274}}
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| * '''[[Localization]]:'''
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| ** see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [http://www.ncbi.nlm.nih.gov/pubmed/16713562 PubMed]
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| ** forms discrete globular foci in germinating spres that colocalize with the nucleoid {{PubMed|24244006}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00880&redirect=T BSU00880]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3C23 3C23] {{PubMed|18439896}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37573 P37573]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00880]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[ctsR]]-[[mcsA]]-[[mcsB]]-[[clpC]]-[[radA]]-[[disA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed]
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| ** ''disA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=disA_107476_108558_1 disA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[ctsR]]'': [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]
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| ** ''disA'': [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
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| * '''Regulation:'''
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| ** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed], induced by heat ([[CtsR]]) [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed]
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| ** expressed during [[sporulation]] in the mother cell {{PubMed|24244006}}
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| ** expressed during germination and spore outgrowth {{PubMed|24244006}}
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| * '''Regulatory mechanism:''' [[CtsR]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 314 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 393 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 465 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 179 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 232 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP987 (''[[disA]]''::''tet''), available in [[Jörg Stülke]]'s lab
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| ** BKG2 (''[[radA]]-[[disA]]::spc''), available in [[Jörg Stülke]]'s lab
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| ** 1A939 ( ''disA''::''tet''), {{PubMed|16713562}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A939&Search=1A939 BGSC]
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| * '''Expression vector:'''
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| ** IPTG inducible expression of His-''disA'' in ''E. coli'': pGP2563 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Fabian Commichau]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 22933559 18714086 25869574 </pubmed>
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| == Original publications ==
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| <pubmed>24244006,8793870,9987115,16713562, 23760274,11544224 17434969 18439896 16713555 12493822 21566650 23192352 23608499 25616256</pubmed>
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| [[Category:Protein-coding genes]]
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