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| * '''Description:''' [[protein kinases and phosphatases|protein kinase]] C, induce [[germination]] of spores in response to DAP-type, and not to Lys-type cell wall muropeptides <br/><br/>
| | {{#bank: PrkC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''prkC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloP ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein kinase]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[germination]] in response to muropeptides
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15770 prkC]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrkC PrkC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=prkC prkC]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 4.833
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1944 bp, 648 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prpC]]'', ''[[cpgA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15770 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:prkC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prkC_1651142_1653088_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:prkC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15770]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[germination]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU15770
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| ===Phenotypes of a mutant ===
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| * unable to germinate in response to muropeptides {{PubMed|18984160}}
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| * reduced growth at high salt {{PubMed|25845974}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15770&redirect=T BSU15770]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13391]
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| === Additional information===
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|
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
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| * '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Proteins phosphorylated by PrkC ===
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| [[CpgA]], [[tufA|EF-Tu]], [[YezB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [[fusA|EF-G]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18984160 PubMed], [[YwjH]], [[GlnA]], [[Icd]], [[AlsD]], [[ptsH|HPr]] {{PubMed|20389117}}, [[YkwC]] {{PubMed|24390483}}, [[YvcK]] {{PubMed|25012659}}, [[GpsB]] {{PubMed|25845974}}
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** contains three C-terminal [[PASTA domain]]s (aa 356-424, 425-492, 493-559) (binds muropeptides) {{PubMed|18984160}}
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| * '''Modification:''' phosphorylation on Thr-290 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], autophosphorylation on multiple threonine residues {{PubMed|12842463,20389117}}
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** activated by muropeptides {{PubMed|18984160}}
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| ** [[GpsB]], [[DivIVA]], and [[EzrA]] are required for stimulation of [[PrkC]] activity {{PubMed|25845974}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[AbrB]]-[[PrkC]] {{PubMed|24731262}}
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| ** [[YvcK]]-[[PrkC]] {{PubMed|25012659}}
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| ** [[GpsB]]-[[PrkC]] {{PubMed|25845974}}
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| * '''[[Localization]]:'''
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| ** inner spore membrane {{PubMed|18984160}}
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| ** membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/12406230 PubMed]
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| ** division septum {{PubMed|25845974}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15770&redirect=T BSU15770]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3PY9 3PY3] (entire extra-cellular region of PrkC from ''Staphylococcus aureus'') {{PubMed|21208192}}
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| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4X3F 4X3F] (intracellular domain of the ''Mycobacterium tuberculosis'' enzyme, 36% identity, 68% similarity) {{PubMed|25586004}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34507 O34507]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15770]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.11.1 2.7.11.1]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[prpC]]-[[prkC]]-[[cpgA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/16025310?ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prkC_1651142_1653088_1 prkC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP576 (spc), OMG302 (aphA3), available in [[Stülke]] lab
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| ** 1A820 ( ''prkC''::''erm''), {{PubMed|12682299}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A820&Search=1A820 BGSC]
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| ** 1A963 (no resistance), {{PubMed|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A963&Search=1A963 BGSC]
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| ** 1A964 (no resistance), {{PubMed|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A964&Search=1A964 BGSC]
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| * '''Expression vector:'''
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| ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP832, available in [[Stülke]] lab
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| ** for expression/ purification of the kinase domain from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP849, available in [[Stülke]] lab
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| ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1001, available in [[Stülke]] lab
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| ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP825, available in [[Stülke]] lab
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| * '''lacZ fusion:''' pGP829 (in [[pAC7]]), available in [[Stülke]] lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20972452 21372323 </pubmed>
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| ==Phosphorylation of PrkC==
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| <pubmed>12842463 20563625</pubmed>
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| ==Targets of PrkC-dependent phosphorylation==
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| <pubmed>19246764, 20070526 ,20389117 24390483 24731262 25012659 25278935 25845974</pubmed>
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| ==Phsiological role of PrkC==
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| <pubmed>12399479, 12406230, 19246764, 12842463 , 18984160 </pubmed>
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| ==Expression of PrkC ==
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| <pubmed>16025310 24752279</pubmed>
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| ==Structure/ biochemistry of PrkC==
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| <pubmed> 21208192 22111897 23793375 25668224 25586004</pubmed>
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| [[Category:Protein-coding genes]]
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