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| * '''Description:''' phosphotransferase of the sporulation initiation [[phosphorelay]] <br/><br/>
| | {{#bank: Spo0F}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spo0F''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase of the sporulation <br/>initiation [[phosphorelay]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37130 spo0F]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Spo0F Spo0F]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=spo0F spo0F]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.697
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 372 bp, 124 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fbaA]]'', ''[[ywjG]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37130 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spo0F_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spo0F_3809550_3809924_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spo0F_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37130]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigH regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37130
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37130&redirect=T BSU37130]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0F.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10411]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| ** phosphorylated on an Asp residue by [[KinA]], [[KinB]], [[KinC]], [[KinD]], or [[KinE]]
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| ** dephosphorylated by [[RapA]], [[RapB]], [[RapE]], or [[RapH]] {{PubMed|17581123}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[Spo0F]]-[[Spo0B]] {{PubMed|12067336}}
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| ** [[Spo0F]]-[[Spo0E]] {{PubMed|12067336}}
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| ** [[RapA]]-[[Spo0F]] {{PubMed|11923303,22267516}}
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| ** [[RapB]]-[[Spo0F]] {{PubMed|12067336}}
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| ** [[RapE]]-[[Spo0F]]
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| ** [[RapH]]-[[Spo0F]] {{PubMed|17581123}}
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| ** [[KinA]]-[[Spo0F]]
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| ** [[KinB]]-[[Spo0F]]
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| ** [[KinC]]-[[Spo0F]]
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| ** [[KinD]]-[[Spo0F]]
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| ** [[KinE]]-[[Spo0F]]
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| ** [[YmcA]]-[[Spo0F]] {{PubMed|23490197}}
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| ** [[RapP]]-[[Spo0F]], dephosphorylation of [[Spo0F]] {{PubMed|23524609}}
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| ** [[RapJ]]-[[Spo0F]], dephosphorylation of [[Spo0F]] {{PubMed|21346797}}
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37130&redirect=T BSU37130]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=1SRR 1SRR] (phosphatase resistant mutant)
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=1F51 1F51] (complex with [[Spo0B]])
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=2JVK 2JVK] (Mutant L66A),
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=2FSP 2FSP]
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| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=3Q15 3Q15] ([[RapH]]-[[Spo0F]] complex) {{PubMed|21346797}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P06628 P06628]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37130]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''spo0F'' {{PubMed|2457578}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spo0F_3809550_3809924_-1 spo0F] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2457578}}, [[SigH]] {{PubMed|1569009}}
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| * '''Regulation:'''
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|8483422}}
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription activation {{PubMed|8483422}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1508 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 750 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>20413551 9299348,9335530,11923303,8608130,16788205,12067336,8497199, 10094672,15299688, 8643670, 8805550, 11069677,2997779,8483422,16333746,2118512, 10997904,9601032,9254596,8730857, 10411754, 17350627, 21346797 9341920 9843420,2509430,1846779,9540996,9477965, 17581123,8483422, 2457578 1569009 23490197 21346797,22267516 23524609 25598361</pubmed>
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| [[Category:Protein-coding genes]]
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