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| * '''Description:''' GTP pyrophosphokinase ([[stringent response]]) <br/><br/>
| | {{#bank: RelA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''relA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || GTP pyrophosphokinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis and degradation of (p)ppGpp, <br/>triggering the [[stringent response]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27600 relA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=relA relA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 84 kDa, 6.964
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2202 bp, 734 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dtd]]'', ''[[apt]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27600 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:relA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=relA_2820529_2822733_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:relA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27600]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[metabolism of signalling nucleotides]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU27600
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| ===Phenotypes of a mutant ===
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| * requirement for valine {{PubMed|24163341}}
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| * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
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| * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant acquires suppressor mutations in ''[[guaA]], [[guaB]], [[gmk]]'' or inactivation of ''[[codY]]'' {{PubMed|24682323,24163341}}
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| * mutation in ''[[relA]]'' results in increased motility and chaining {{PubMed|25331430}}
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| * ''[[relA]]'' mutation increases flagellin production via elevated [[SigD]] level {{PubMed|25331430}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27600&redirect=T BSU27600]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/relA-yrvI.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12665]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)
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| * '''Protein family:''' relA/spoT family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** N-terminal ppGpp hydrolase domain {{PubMed|24163341}}
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| ** central ppGpp synthetase domain {{PubMed|24163341}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27600&redirect=T BSU27600]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O54408 O54408]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27600]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.6.5 2.7.6.5]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[relA]]-[[dtd]]'' {{PubMed|9383190}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=relA_2820529_2822733_-1 relA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 244 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 573 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' ''[[relA]]'' mutant {{PubMed|9383190}} - note that ''[[relA]]'' mutants are prone to suppressor mutations in the ''[[ywaC]]'' or ''[[yjbM]]'' loci {{PubMed|18670626}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
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| Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8 ResearchGate-Profile]
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| =Your additional remarks=
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| =References=
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| <pubmed>25331430,11948165,9383190,10209741,13129942, 24489751, 19447912 13129942 12372825 12081964 11948165 12419222 23028324 24163341 24682323</pubmed>
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| [[Category:Protein-coding genes]]
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