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| * '''Description:''' succinyl-CoA synthetase (beta subunit) <br/><br/>
| | {{#bank: SucC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sucC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || succinyl-CoA synthetase (beta subunit)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16090 sucC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SucC SucC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sucC sucC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 4.846
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1155 bp, 385 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylqH]], [[sucD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16090 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sucC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sucC_1680431_1681588_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sucC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16090]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ATP synthesis]]}},
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}},
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| [[most abundant proteins]]
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16090
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16090&redirect=T BSU16090]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sucCD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12680]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
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| * '''Protein family:''' ATP-grasp domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Reversible Michaelis-Menten [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01%2F01%2F1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234938%231985%23998209998%23270526%23FLP%23display%23Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
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| * '''[[Domains]]:'''
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| * '''Modification:''' phosphorylation on Ser-220 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** Inhibited by 2-oxoglutarate, ATP and NADH [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01%2F01%2F1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234938%231985%23998209998%23270526%23FLP%23display%23Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
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| *** GTP is not accept by the enzyme [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01%2F01%2F1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234938%231985%23998209998%23270526%23FLP%23display%23Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[Mdh]]-[[SucC]] {{PubMed|20933603}}
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| ** [[SucC]]-[[SucD]] {{PubMed|20933603}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16090&redirect=T BSU16090]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1JKJ 1JKJ] (E. coli)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P80886 P80886]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16090]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.5 6.2.1.5]
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| === Additional information===
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| * extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at [http://www.proteopedia.org/wiki/index.php/Succinyl-Coa_Synthetase Proteopedia]
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| =Expression and regulation=
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| * '''Operon:''' ''[[sucC]]''-''[[sucD]]'' {{PubMed|11976317}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sucC_1680431_1681588_1 sucC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' repressed by glucose (2.7-fold) ([[CcpA]]) {{PubMed|12850135}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]: transcription repression {{PubMed|12850135}}
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| ** [[RoxS]]: inhibition of translation and initiation of RNA degradation by [[rnc|RNase III]] {{PubMed|25643072}}
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| * '''Additional information:'''
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| ** the mRNA base-pairs with the [[RoxS]] [[sRNA]] {{PubMed|25643072}}
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| ** the ''[[roxS]]-[[sucC]]'' RNA duplex forms a cleavage site for [[rnc|RNase III]] {{PubMed|25643072}}
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| ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 7352 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 44785 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 10243 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 5100 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 8473 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A1006 ( ''sucC''::''spec''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1006&Search=1A1006 BGSC]
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| ** GP1134 (cat), available in [[Jörg Stülke]]'s lab
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| ** GP791 (''[[sucC]]-[[sucD]]''::''tet''), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12850135 17218307 11976317 20933603 15378759 22900538 25643072 </pubmed>
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| [[Category:Protein-coding genes]]
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