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* '''Description:''' HPr, General component of the sugar phosphotransferase system ([[PTS]]).  <br/><br/>
{{#bank: PtsH}}


{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptsH''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || histidine-containing phosphocarrier <br/>protein HPr of the [[PTS]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || [[PTS]]-dependent sugar transport <br/>and carbon catabolite repression
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13900 ptsH]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtsH PtsH]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ptsH PtsH]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9,1 kDa, 4.58
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 264 bp, 88 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptsG]]'', ''[[ptsI]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13900 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13900 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13900 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ptsH_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsH_1459384_1459650_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ptsH_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13900]]
|-
|}
__TOC__
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= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[phosphoproteins]]}},
[[most abundant proteins]]
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[GlcT regulon]]}},
{{SubtiWiki regulon|[[stringent response]]}}
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU13900
===Phenotypes of a mutant ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13900&redirect=T BSU13900]
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptsGHI.html]
* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10200]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:''' Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein [[PtsH|HPr]] + protein EIIA N(tau)-phospho-L-histidine
* '''Protein family:''' HPr domain (according to Swiss-Prot) HPr family
* '''Paralogous protein(s):''' [[Crh]]
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''[[Domains]]:''' HPr Domain (2–88)
* '''Modification:'''
** transient phosphorylation by [[PtsI |Enzyme I]] of the PTS on His-15
** regulatory phosphorylation on Ser-46 by [[HprK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2507315 PubMed]
** an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537  PubMed]
** weak phosphorylation on Ser-12 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
** ''in vitro'' phosphorylated by [[PrkC]] on Ser-12 {{PubMed|20389117}}
* '''[[Cofactors]]:'''
* '''Effectors of protein activity:'''
* '''[[SubtInteract|Interactions]]:'''
** [[HprK]]-[[PtsH|HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12009882 PubMed]
** [[PtsI|Enzyme I]]-[[PtsH|HPr]]
** [[PtsH|HPr]]-[[PtsG]] {{PubMed|8418852}},  [[PtsH]]-[[MtlF]] {{PubMed|20444094}}, [[ManP]]-[[PtsH|HPr]], [[GmuA]]-[[PtsH|HPr]]
** [[GamP]]-[[PtsH|HPr]], [[BglP]]-[[PtsH|HPr]], [[LicA]]-[[PtsH|HPr]], [[LevD]]-[[PtsH|HPr]]
** [[FruA]]-[[PtsH|HPr]], [[YpqE]]-[[PtsH|HPr]]
** [[PtsH|HPr]]-[[LicT]],  [[PtsH|HPr]]-[[SacY]],  [[PtsH|HPr]]-[[SacT]],  [[PtsH|HPr]]-[[GlcT]]
** [[PtsH|HPr]]-[[MtlR]] {{PubMed|20444094}}, [[PtsH|HPr]]-[[LicR]], [[PtsH|HPr]]-[[LevR]],[[PtsH|HPr]]-[[ManR]]
** [[PtsH|HPr]]-[[GlpK]]
** [[GapA]]-[[PtsH|HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17142398 PubMed]
** [[PtsH|HPr(Ser-46)-P]]-[[CcpA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12432959 PubMed]
** [[PtsH|HPr]]-[[RbsR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16519689 PubMed]
** [[PtsH|HPr(His)-P]]-[[RhgR]] {{PubMed|19651770}}
* '''[[Localization]]:''' cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16395550 PubMed]
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13900&redirect=T BSU13900]
* '''Structure:'''
** [http://www.rcsb.org/pdb/explore.do?structureId=1KKM 1KKM] (complex of ''L. casei'' [[HprK]] with ''B. subtilis'' HPr-Ser-P)
**  [http://www.rcsb.org/pdb/explore.do?structureId=1KKL 1KKL] (complex of ''Lactobacillus casei'' [[HprK]] with ''B. subtilis'' HPr) 
** [http://www.rcsb.org/pdb/explore.do?structureId=2HID 2HID] (NMR)
** [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3OQM 3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[[PtsH |HPr]] and the ''[[ackA]]'' operator site)
** [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3OQN 3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[[PtsH |HPr]] and the ''[[gntR]]'' operator site)
** [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3OQO 3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[[PtsH |HPr]] and a optimal synthetic operator site)
* '''UniProt:''' [http://www.uniprot.org/uniprot/P08877 P08877]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13900]
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.11.- 2.7.11.-]
=== Additional information===
=Expression and regulation=
* '''Operon:'''
** ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}
**''[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsH_1459384_1459650_1 ptsH] {{PubMed|22383849}}
* '''[[Sigma factor]]:'''
** ''[[ptsG]]'': [[SigA]] {{PubMed|11902727}}
** ''[[ptsH]]'': [[SigA]] {{PubMed|11902727}}
* '''Regulation:'''
** expression activated by glucose (2 fold) ([[GlcT]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
** the ''[[ptsH]]'' promoter is constitutive {{PubMed|11902727}}
** subject to negative stringent control upon amino acid limitation (due to control of'' [[ptsG]]'' transcription initiation) {{PubMed|20081037}}
* '''Regulatory mechanism:'''
** ''[[ptsG]]'': transcriptional antitermination via the [[GlcT]]-dependent [[RNA switch]] {{PubMed|9765562}}
* '''Additional information:'''
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 352 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 7193 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 914 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5795 {{PubMed|21395229}}
=Biological materials =
* '''Mutant:''' available in [[Jörg Stülke]]'s lab:
** MZ303 (cat)
** GP507 ptsH1 (S46A)
** GP506 (ptsH-H15A)
** GP777 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette), {{PubMed|22722928}}
* '''Expression vector:'''
** pGP438 (with N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab
** pAG2 (His-tag) {{PubMed|9237995}}, available in [[Anne Galinier]] lab
** pGP371(expression / purification of HPr-S46A, with His-tag from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP1415 (HPr, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Jörg Stülke]]'s lab
** pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], available in [[Jörg Stülke]]'s lab
** pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Jörg Stülke]]'s lab
** pGP2431 (N-terminal Strep-tag, expression and purification from ''B. subtilis'', in [[pGP380]]), for [[SPINE]], available in [[Jörg Stülke]]'s lab
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''CFP fusion:''' B. subtilis GP1267 ptsH-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
* '''Antibody:''' available in [[Jörg Stülke]]'s lab
=Labs working on this gene/protein=
[[Josef Deutscher]], Paris-Grignon, France
[[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[[Wolfgang Hillen]], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
[[Richard Brennan]], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
[[Boris Görke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]
[[Anne Galinier]], University of Marseille, France
=Your additional remarks=
=References=
<pubmed>16519689,,12850135 17218307 16519689 17142398 12359875 1577686 9162046 11929549 9336834 7803390, 7623661, 2846556, 8169206, 9973552, 15126459, 10048041, 12169607, 9622354, 10217795, 17693724, 18757537 , 9202047 , 7592487, 15369672 , 14527945, 2507315 8580838 19651770 8418852 1303754 1549615 20081037 20389117 20444094 22001508 22722928 23551403 15378759</pubmed>
[[Category:Protein-coding genes]]

Latest revision as of 12:35, 20 August 2015

PtsH

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