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| * '''Description:''' protein component of [[RNase]] P, metal-dependent 5' end maturation of precursor [[tRNA]]s <br/><br/>
| | {{#bank: RnpA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rnpA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || protein component of [[RNase]] P (substrate specificity)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || cleavage of precursor sequences <br/>from the 5' ends of pre-[[tRNA]]s
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU41050 rnpA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RnpA RnpA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 10.804
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 348 bp, 116 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIIJ]]'', ''[[rpmH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU41050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU41050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU41050 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ThdF_jag_spoIIIJ_rnpA_rpmH_context.png]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnpA_4214753_4215103_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rnpA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU41050]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[Rnases]]}},
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU41050
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU41050&redirect=T BSU41050]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10063]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from [[tRNA]] precursor (according to Swiss-Prot)
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| ** 5' end maturation of tmRNA {{PubMed|25402410}}
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| * '''Protein family:''' rnpA family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** RNR motif: residues 60-68, stabilizes complexes with both the RNA part of [[RNase]] P (''[[rnpB]]'') and with pre-[[tRNA]] ([[rnpB]]-[[RnpA]]-pre-[[tRNA]]) {{PubMed|21622899}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** forms dimers {{PubMed|21764917}}
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| ** [[RnpA]]-[[RpmH]]
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| ** [[RnpA]]-[[RnpB]] {{PubMed|21622899}}
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| ** [[RnpA]]-[[CshA]] {{PubMed|21764917}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU41050&redirect=T BSU41050]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A6F 1AF6] [http://www.ncbi.nlm.nih.gov/sites/entrez/9563955 PubMed]
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| ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3OKB 3OKB] (complex of RnpA with the RNA [[RnpB]] and [[tRNA]], from ''Thermotoga maritima'') {{PubMed|21076397}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P25814 P25814]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU41050]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.26.5 3.1.26.5]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnpA_4214753_4215103_-1 rnpA] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| ** expression transiently increases in the forespore {{PubMed|22848659}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Carol Fierke]], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
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| * [[Roland Hartmann]], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 19215776 8901495 17868095 14691942 16650980 16980936 16679018 14550630 </pubmed>
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| ==Original Publications==
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| <pubmed>10390342,17919279,17355991,11812129,16980484,11454206, 19549719,11454206 9563955, 19932118 17299131 20476778 11258888 16185070 21764917 22848659 21076397,20843005,21622899 24364358 25249623 25402410</pubmed>
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| [[Category:Protein-coding genes]]
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