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| * '''Description:''' [[two-component systems|two-component]] response regulator ([[transcription factors of the OmpR family|OmpR family]]), controls cell wall metabolism <br/><br/>
| | {{#bank: WalR}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''walR''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yycF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] response regulator ([[transcription factors of the OmpR family|OmpR family]])
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of cell wall metabolism
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40410 walR]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=WalR WalR]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 4.876
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 705 bp, 235 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[walK]]'', ''[[trnY-Phe]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40410 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yycF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walR_4153696_4154403_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:walR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40410]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall/ other]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The [[WalR regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40410
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| ===Phenotypes of a mutant ===
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| * essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| * a constitutively active form of [[WalR]] ([[WalR]]-R204C) results in constitutive expression of the [[WalR]] regulon, and in combination with a ''[[sepF]]'' mutation to the formation of cell wall-less L-forms {{PubMed|22122227}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40410&redirect=T BSU40410]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yycFGHIJ-yyxA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10001]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' [[transcription factors of the OmpR family|OmpR family]] of two-component response regulators
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:''' phosphorylated by [[WalK]] on an Asp residue
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:''' phosphorylation likely affects DNA-binding activity
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[WalK]]-[[WalR]]
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40410&redirect=T BSU40410]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2D1V 2D1V] (DNA binding domain), [http://www.rcsb.org/pdb/explore.do?structureId=2ZWM 2ZWM] (receiver domain)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37478 P37478]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40410]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[walR]]-[[walK]]-[[yycH]]-[[yycI]]-[[walJ]]-[[htrC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9829949 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walR_4153696_4154403_-1 walR] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** expressed during vegetative growth, repressed during stationary phase [http://www.ncbi.nlm.nih.gov/sites/entrez/9829949 PubMed]
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| ** induced at high temperature {{PubMed|24125693}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 176 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** pGP2633, constitutive expression in ''B. subtilis'' (based on [[pBQ200]]), available in [[Jörg Stülke]]'s lab
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| ** pGP2635, expression of His-[[WalR]] in ''E. coli'' (based on [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Kevin Devine]], Dublin
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19019149, 18245295,18792692, 22126136, 10094672 </pubmed>
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| ==The [[WalR regulon]]==
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| <pubmed>17581128,10878122,12950927, 23199363</pubmed>
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| ==Other original Publications==
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| <pubmed>18573169,17350627,10094672,17827301,9829949,16030236,19574649, 20059685 20111065 20167622 20834167 24125693 22122227 24125693 24490131 18789936 </pubmed>
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| [[Category:Protein-coding genes]]
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